Core Facilities

Center for Cell-Based Therapy maintains a variety of several core facilities to support ours research programs and educational activities, including laboratories of Cell Therapy, Molecular Biology, Gene Transfer, Molecular Genetic Analysis, Flow Cytometry, Bioinformatics, Protein Chemistry, and HLA Genotyping. The CTC core facilities are staffed by highly experienced researchers in the technologies offered.

Animal Manipulation Core Bionformatics Cell Culture
Cell Therapy Core Confocal and Microscopy DNA sequencing
Domestic Animal Facility Embryo Micromanipulation Flow Cytometry
Hematologia Experimental HLA Genotyping Library
Mass Spectrometry Microarray Lab Next Generation Sequencing (NGS)
High Content Screening (HCS)  

 

Experimental Hematology Laboratory The Experimental Hematology Laboratory works at the Hospital of Ribeirão Preto Medical School (USP) and the Blood Center of Ribeirão Preto, working in the diagnostics and research in blood diseases. Link for the site.
Flow Cytometry Lab Through the flow cytometry technique the laboratory performs testing such as quantification lymphocyte subpopulations of HIV patients, progenitor stem cells quantification for allogeneic or autologous transplant, platelet syndromes determination, peripheral blood subpopulation quantification, and other methodologies. This technique allows the measurement of the chemical and physical characteristics in particles or in cells in suspension. The laboratory also supports researches performed on mesenchymal, endothelial, and dendritic cells. In order to follow the technological advances, the Hemocentro has recently bought the Flow Cytometer, which is able to perform the “cell sorter” (separation of subpopulation), to perform simultaneous analyses on seven different fluorescence, and so forth.
Cell Culture Core Facility Our goal is to provide technical support to Center for Cell-Based Therapy researchers in projects to adult and embryonic stem cells. We also conduct basic research with human pluripotent and multipotent stem cells. Our major focus is the generation, in vitro, of hematopoietic cells and endothelial from human embryonic (hESCs) and induced pluripotent stem cells (hiPS). These studies are used to characterize molecular and cellular mechanism underpinning the differentiation of hematopoietic progenitors and mature hematopoietic cells. This research will contribute to clinical translation of hESC- and iPSC-derived hematopoietic cells for novel therapies in the next few years. The laboratory also performs researches on multipotent mesenchymal stromal cells (MSCs). These studies comprise MSCs isolation, culture, expansion and characterization, contributing for a better understanding of the potential clinical usage of these cells.
Cell Therapy Core Facility Our goal is to provide Hematopoietic (HSCs) and Mesenchymal stem cells (MSCs) for clinical researchers performed by the Center for Cell-Based Therapy.  We have the expertise to manipulate and cryopreserve HSCs for transplantation since 1997. In our facility, the products which contain hematopoietic stem cell (HSC) such as bone marrow, umbilical cord blood and mobilized peripheral blood are processed in order to keep their biological function until the moment of usage, which can happen years after the collection. We also expanded MSC in relative large scale in culture system using T flask. The MSCs are tested for safety (sterility, mycoplasma, adventitious virus), identity/purity (morphology, antigenic phenotype and cell viability by flow cytometry), potency to inhibit T lymphocyte proliferation and to differentiate into adipocytes and osteocytes, purity (endotoxin) and stability (consistent composition, stable karyotype [G-Banding] and ability to differentiate and to inhibit T lymphocyte proliferation) after long term culture. Our next step is to create allogeneic MSC cell bank, where the cryopreserved banked cells will be previously tested, thus ready for “off-the-shelf” clinical use.
Animal Manipulation Core This unit is SPF (specific pathogen-free). It is composed of 3 rooms for mice and rats. It has the basic equipment: iron shelves and a “pass-through”, autoclaves, ventilated breeding racks, shelves for maintenance of approximately 300 couples of matrixes of mice in expansion, a total of 8 lines, providing the scientific community an average of 500 mice per month and a total of 40 couples (matrixes) of Wister rats.
Mass Spectrometry and Protein Chemistry Lab The Protein Chemistry Center (CQP) provides scientific and technical support for proteomics and mass spectrometry analysis. The Center for proteomics and mass spectrometry is equipped with three mass spectrometer (ESI-Triple Quadrupole, ESI-Q-TOF and MALDI-TOF/TOF) and complete systems for on line and off line protein/peptide separation by capillary liquid chromatography, specialized software for protein quantification and identification using local or internet databases.
Bioinformatics Team The Bioinformatic Team (BiT) provides scientific and technical support for molecular biology, genomics, proteomics and other biological related approaches. The BiT Lab is equipped with high-performance computer server, specialized software, and advanced database systems to genomic analysis.
DNA sequencing and Next Generation Sequencing Lab The High-Throughput DNA and RNA Sequencing facility provides access to automated DNA sequencing technology. The services offered include experimental design consultation, library preparation, data export, and primary and secondary data analysis.
Confocal and Microscopy Confocal Microscopy Laboratory is a facility designed to serve as a service center for researchers in need of primary stem cells, tumor cells from either human or murine analyses. We are specialized in morphology characterization, detection of specific proteins by immunofluorescence and detection of fluorescence-labelled cells. We study cultured normal and tumor cells and tissue samples from either human or animal models. The core is equipped with Laser Scanning Microscopy (LSM710, Carl Zeiss).
Microarray Lab Equipped with an Agilent auto-loader High-Density Microarray Scanner, an Axon GenePix 4000B (Molecular Devices), and all the additional processing hardware; the trained specialists in the facility can generate data derived from Agilent or any other microarrays type (including antibody or protein microarrays). Among the possibilities, the expression levels of over 27,958 Entrez Gene mRNAs and 7,419 lincRNAs can be evaluated simultaneously or, alternatively, the entire set of microRNAs from the Sanger miRbase (8x60K microarrays). Moreover, DNA gains or losses can be evaluated down to a resolution of around 2 KB, along the whole genome (1x1M CGH microarrays). Finally, the use of promoter or CpG focused microarrays, coupled to Chromatin Immunoprecipitation experiments, allows the study of promoter occupancy by Transcription Factors (ChIp-chip) or the epigenomic profiling of DNA modifications (Methyl-chip).
HLA Genotyping Lab The Human Leukocyte Antigen (HLA) Laboratory provides histocompatibility testing for kidney and bone marrow transplants, working 24h a day. Donor-recipient pairs are analyzed for HLA antigens compatibility. The HLA Laboratory performs compatibility testing for: 1, all cadaver donors of the São Paulo State; 2, living kidney and bone marrow donors (family members) for transplantation in the University Hospital of School of Medicine of Ribeirão Preto, University of São Paulo; 3, volunteer donor banks of bone marrow and umbilical cord blood, REDOME and Brasilcord respectively, which are centralized at the “Instituto Nacional do Câncer of Rio de Janeiro”.
Embryo Micromanipulation The Embryo Micromanipulation lab have a complete structure for in vitro production of embyos in a broad range of domestic species, including equipments for embryo and gamete micromanipulation (compose of inverted epifluorescence microscope equipped with micromanipulators, Narishige microinjectors, FemtoJet® microinjector, electrofusion system, Piezo Drill, besides the equipment’s to produce borosilicate tools); as well as embryo and cell in vitro culture systems with controlled atmosphere.
Domestic Animal Facility This facility is an experimental Animal Hospital expected to be ready for use on July 2012, created to serve as a Laboratory for teaching and for doing research on innovative therapies (INOVATERAP) financed by FINEP.
This experimental facility will have 3,000 m2 infrastructure for large and small domestic animal care, clinic, image and lab. analysis, as well as surgery and pathology. Besides of the hospital facility a 143 m2 lab. will be available to produce and handle cells Germ free condition.
Library Library: (42 m²): this unit have 1,160 books, 4,000 periodic and journals, 19 references sets (printed and digitalized), thesis, dissertations and monographs, guides, CAPES electronic signatures, 3 journals signatures, annals and collection of scientific production of local researchers.
Next Generation Sequencing (NGS) For the investigation of genetic and epigenetic changes. The epigenomic signatures are obtained by large-scale or new-generation sequencing. The experience of the group with the analysis of data generated by large-scale sequencing has permitted the development of a new bioinformatic tool called biOMICS (Biologically Integrating Omics), whose objective is to integrate information from the projects ENCODE, NIH Roadmap and The Cancer Genome Atlas (TCGA) with new large-scale sequencing data. These activities are coordinated by Houtan Noushmehr, a collaborator linked to the group coordinated by Wilson Araújo da Silva Junior (PI).
  High Content Screening (HCS) HCS recently emerged as a powerful tool for the functional screening of genomic libraries. Our group recently acquired an HCS apparatus for the study of the role of microRNAs in the maintenance of pluripotentt status and stem cell differentiation.