Andréia M. Leopoldino
PhD Professor of Pathophysiology of human metabolic diseases, FCFRP/USP
andreiaml@usp.br
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Education 1993-1998 Graduate in Biological Sciences, Federal University of Minas Gerais, Brazil
1999-2000 Master Thesis in Biochemistry, Institute of Biological Sciences, Federal University of Minas Gerais, Brazil
2000-2002 PhD in Biochemistry, Institute of Biological Sciences, Federal University of Minas Gerais, Brazil
2003-2006 Postdoctoral Research Fellow, University of the State of Sao Paulo, Brazil
2006-2006 Postdoctoral Research Fellow, National Institutes of Health, Bethesda, MD, USA
Research Interests Human and Medical Genetics
Biochemistry
Molecular Oncology
Cell Signaling
Research Overview The focus of our research is on the understanding of the molecular basis of signal transduction in cell proliferation, invasion, differentiation and neoplastic transformation, and on the use of this knowledge to propose new therapeutic targets and prognostic markers. Proteomic and gene expression strategies are applied to identify and characterize signaling pathways controlled by two protein markers in cancer. Another area is the identification and functional characterization of miRNAs involved in apoptosis and metastasis which contribute to resistance of tumor cells, consequently affecting the survival of cancer patients. For this study, we are applying PCR array systems for genes and miRNA expression and also miRNA microarray to obtain the profile of expression in cell lines with the cancer marker overexpressed or down-regulated by siRNA or shRNA. Epigenetic alterations are also identified using PCR array systems. A xenograft model in nude mice has been adopted to investigate the role of these markers in head and neck carcinoma.
Lab Staff Lays Martin Sobral,Post-Doc/FAPESP
Camila Sayuri Matsumoto, PhD student/FAPESP
Karina Stringhetta, PhD student/FAPESP
Renata Nishida Goto, PhD student/FAPESP

Molecular and Cellular Biology Technician
Cristiana B. Garcia, PhD and Lucas de Oliveira Sousa, PhD

Collaborators Lewis J. Greene
Centro de Química de Proteínas, Regional Blood Center of Ribeirão Preto, SP
ljgreene@fmrp.usp.br

Eduardo M. Rego
Regional Blood Center of Ribeirão Preto, SP
emrego@hcrp.usp.br

Carlos Curti
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, SP
ccurti@fcfrp.usp.br

Wilson A. Silva-Jr.
Regional Blood Center of Ribeirão Preto, SP
wilsonjr@usp.br

International Collaborators Silvio J. Gutkind, PhD
Chief, Oral and Pharyngeal Cancer Branch, DIR
Chief, Cell Growth Regulation Section, and Molecular Carcinogenesis Unit, OPCB, DIR
BUILDING 30 ROOM 211
30 CONVENT DR MSC 4340
BETHESDA MD 20892-4340

Cristiane H. Squarize, PhD
Assistant Professor
Division of Oral Pathology/Medicine/Radiology
Department of Periodontics and Oral Medicine
University of Michigan School of Dentistry
1101 N. University Ave Rm #3210
Ann Arbor, MI 48109-1078

Facilities Coordination
Selected Publications Lays M Sobral, Lucas O Sousa, Ricardo D Coletta, Hamilton Cabral, Lewis J Greene, Eloiza H Tajara, J Silvio Gutkind, Carlos Curti, Andréia M Leopoldino. Stable SET knockdown in head and neck squamous cell carcinoma promotes cell invasion and the mesenchymal-like phenotype in vitro, as well as necrosis, cisplatin sensitivity and lymph node metastasis in xenograft tumor models. Molecular Cancer, v.13, p.32, 2014.Luciana O Almeida, Cristiana B Garcia, Flavia A Matos-Silva, Carlos Curti, Andréia M Leopoldino. Accumulated SET protein up-regulates and interacts with hnRNPK, increasing its binding to nucleic acids, the Bcl-xS repression, and cellular proliferation. Biochemical and biophysical research communications, v.445, p.196-202, 2014.

Luciana O Almeida, Aline C Abrahao, Luciana K Rosselli-Murai, Fernanda S Giudice, Chiara Zagni, Andreia M Leopoldino, Cristiane H Squarize, Rogerio M Castilho. NFκB mediates cisplatin resistance through histone modifications in head and neck squamous cell carcinoma (HNSCC). FEBS Open Bio, v.4, 96-104, 2014.

Luciana O Almeida, Renata N Goto, Cezar R Pestana, Sergio A Uyemura, Silvio Gutkind, Carlos Curti, Andreia M Leopoldino. SET overexpression decreases cell detoxification efficiency: ALDH2 and GSTP1 are downregulated, DDR is impaired and DNA damage accumulates. FEBS Journal, v.279, p. 4615-4628, 2012.

Andréia M Leopoldino, Cristiane H Squarize, Cristiana B Garcia, Luciana O Almeida, Cezar R Pestana, Lays M Sobral, Sergio A Uyemura, Eloiza H Tajara, J Silvio Gutkind, Carlos Curti. SET protein accumulates in HNSCC and contributes to cell survival: Antioxidant defense, Akt phosphorylation and AVOs acidification. Oral Oncology, v.48, 1106-1113, 2012.

Andréia M Leopoldino, Cristiane H Squarize, Cristiana B Garcia, Luciana O Almeida, Cezar R Pestana, Ana CM Polizello, Sérgio A Uyemura, Eloiza H Tajara, J Silvio Gutkind, Carlos Curti. Accumulation of the SET protein in HEK293T cells and mild oxidative stress: cell survival or death signaling. Molecular and cellular biochemistry, v.363, p.65-74, 2012.

Giovana M Polachini, Lays M Sobral, Ana MC Mercante, Adriana F Paes-Leme, Flávia CA Xavier, Tiago Henrique, Douglas M Guimarães, Alessandra Vidotto, Erica E Fukuyama, Jose F Gois-Filho, Patricia M Cury, Otavio A Curioni, Pedro Michaluart Jr, Adriana MA Silva, Victor Wünsch-Filho, Fabio D Nunes, Andréia M Leopoldino, Eloiza H Tajara. Proteomic approaches identify members of cofilin pathway involved in oral tumorigenesis. Plos One, v.7(12), e50517.

BARBIERI, M.R.; ANDRADE, C.D.C.; SILVA, W.A.; MARQUES, A.A.; LEOPOLDINO, AM; MONTES, M.B.A.; Dias-Baruffi, M.; SOARES, I.C.; WAKAMATSU, A.; ALVES, V.A.; LAURE, H.J.; Zago, M.A.; GREENE, L.J. Expression of human protein S100A7 (psoriasin), preparation of antibody and application to human larynx squamous cell carcinoma. BMC Research Notes, v. 4, p. 494, 2011.

PARDO-ANDREU, G.L.; NUNEZ-FIGUEREDO, Y.; TUDELLA, V.G.; CUESTA-RUBIO, O.; Rodrigues, F.P.; PESTANA, C.R.; Uyemura, S.A.; LEOPOLDINO, A.M.; ALBERICI, L.C. ; CURTI, C. The anti-cancer agent nemorosone is a new potent protonophoric. Mitochondrion (Amsterdam. Print), v.11; pp.255-263, 2011.

Barreto, V.; LEOPOLDINO, AM; TAFT, C.A.; Tomich CHPS. Toxicophoric and Metabolic In Silico Evaluation of Benzimidazole and Phenylbenzamide Derivatives with Potential Application as Anticancer Agents. Drug Metabolism Letters LTD, v. 5, p. 267-275, 2011.
CARREGARO, F.; CARTA, A.; LOBO, S.A.; TAJARA, E.H.; LEOPOLDINO, A.M. Polymorphisms IL10-819 and TLR-2 are potentially associated with sepsis in Brazilian patients. Memórias do Instituto Oswaldo Cruz (Online), v. 105, p. 949-956, 2010.

RODRIGUES-LISONI, F.C.; PEITL, P.; VIDOTTO, A.; POLACHINI, G.M.; MANIGLIA, J.V.; CARMONA-RAPHE, J.; CUNHA, B.R.; HENRIQUE, T.; SOUZA, C.F.; TEIXEIRA, R.A.P.; FUKUYAMA, E.E.; MICHALUART, P.; de CARVALHO, M.B.; OLIANI, S.M.; HEAD AND NECK GENCAPO*; TAJARA, E.H. Genomics and proteomics approaches to the study of cancer-stroma interactions. BMC Medical Genomics, v. 3, p. 14, 2010.

SILVEIRA, N.J.F.; VARUZZA, L.; MACHADO-LIMA, A.; LAURETTO, M.; PINHEIRO, D.G.; RODRIGUES, R.V.; SEVERINO, P.; NOBREGA, F.G.; HEAD AND NECK GENCAPO*; LEOPOLDINO, A.M.; SILVA JR., W.A.; PEREIRA, C.A.B.; TAJARA, E.H. Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and ioinformatics analysis of larynx-derived SAGE libraries. BMC Medical Genomics, v. 1, p. 56, 2008.

LEOPOLDINO, AM, CARREGARO, F., Tomich CHPS, Feitosa AO, MANCINI, U. M., FREITAS, J. M., Tajara, E. H. S. Sequence and transcriptional study of HNRPK pseudogenes, and expression and molecular modeling analysis of hnRNP K isoforms. Genome (Ottawa). V.50(5); pp.451-462, 2007.

Anacleto C, LEOPOLDINO, AM, ROSSI, B., SOARES, F.A., LOPES, A., ROCHA, J. C. C., CABALLERO, O., CAMARGO, A., SIMPSON, A., PENA, S.D.J. Colorectal cancer methylator phenotype: fact or artifact. Neoplasia. , v.7, p.331 – 335, 2005.

Bavoux C, LEOPOLDINO, AM, Ogi T, Bieth A, Kumari A, Lehmann A, PENA, S.D.J., Renato Machado C, Hoffmann JS, Cazaux C. Misregulation of the error-prone DNA polymerase kappa promotes pleiotropic genetic disorders and tumorigenesis. Cancer Research. , v.65, p.325 – 330, 2005.

LEOPOLDINO, AM, PENA, S.D.J. The mutational spectrum of human autosomal tetranucleotide microsatellites. Human Mutation. , v.21, p.71 – 79, 2003.

LEOPOLDINO, AM, PENA, S.D.J. The mutational spectrum of human autosomal tetranucleotide microsatellites. Human Mutation. , v.21, p.71 – 79, 2003.