Simone Kashima Haddad
Researcher, Regional Blood Center of Ribeirão Preto, SP
skashima@hemocentro.fmrp.usp.br
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Education   1988-1991 Graduated in Pharmaceutical Sciences, University of São Paulo, Ribeirão Preto, Brazil
1992-1996 Master in Basic and Applied Immunology, at Medical School of Ribeirão Preto, University of São Paulo
2000-2004 PhD in Clinical Medicine, Medical School of Ribeirão Preto, University of São Paulo
2006-2008 Post Doctorate in Medical School of Ribeirão Preto, University of São Paulo
Research Interests  

Epithelial Mesenchymal Transition (EMT), Endothelial Mesenchymal Transition (EnMT)
Induced pluripotent stem cell
Infectious diseases
Genomics, Molecular Diagnosis

Research Overview   The major focus of our group is to study molecular mechanisms related to the pluripotency maintenance of stem cells, specially induced pluripotent stem cells (iPS). Understanding these mechanisms, we will provide fundamental insights on developmental biology and in the potential applications on regenerative medicine of these cells. As an important mechanism that is related to stemness, our group is also interested in the Epithelial Mesenchymal Transition (EMT) that is a complex phenomenon involved in embryogenesis, and cancer progression. A specialized form of EMT, Endothelial Mesenchymal Transition (EnMT) is our focus, mainly to understand and characterize the biological properties of endothelial cells.

Another research area in Molecular Biology Lab is to develop molecular platforms applied to diagnosis and to study molecular characteristics of viruses that are involved on transfusion- transmitted diseases such as HIV, HTLV, parvovirus B19. Cytomegalovirus and mycoplasma are also detected for cell therapy in accordance with GMP and hemotherapy needs.

Lab Staff  

Molecular Biology Group
Evandra Strazza Sandoval, MSc PhD Student, Technician
Virginia Mara de Deus Wagatsuma, PhD PhD, Technician
Maria Augusta Sartori Post-Doc/CNPq
Svetoslav Nanev Slavov Post-Doc/FAPESP

Tathiane Maistro Malta Pereira, MSc PhD Student
Mauricio C Rocha Junior PhD Student
Mariana Tomazini Pinto PhD Student
Katia Kaori Otaguiri MSc Student
Mayra Dorigan MSc Student

Collaborators   Bernardo Galvão Castro
Fundação Osvaldo Cruz (FIOCRUZ-BA)
Laboratório Avançado de Saúde Pública

Ricardo Pasquini
Universidade Federal do Paraná
Setor de Ciências da Saúde – Departamento de Clínica Médica

Lúcia Helena Faccioli
Universidade de São Paulo
Faculdade de Ciências Farmacêuticas de Ribeirão Preto
Departamento de Análises Clínicas Toxicológicas e Bromatológicas.

Ivone Carvalho
Universidade de São Paulo
Faculdade de Ciências Farmacêuticas de Ribeirão Preto
Departamento de Ciências Farmacêuticas

Fabíola Attie de Castro
Universidade de São Paulo
Faculdade de Ciências Farmacêuticas de Ribeirão Preto
Departamento de Análises Clínicas Toxicológicas e Bromatológicas

Fabiane Gai Frantz
Universidade de São Paulo
Faculdade de Ciências Farmacêuticas de Ribeirão Preto
Departamento de Análises Clínicas Toxicológicas e Bromatológicas

International Collaborators  

Robert A Weinberg, PhD
Professor of Biology
Whitehead Institute for Biomedical Research
Massachusetts Institute of Technology
http://web.wi.mit.edu/weinberg/pub/ 

Facilities Coordination  

Quantitative Real Time PCR (ABI 7500 SDS Systems)
Molecular Diagnosis for HIV and HTLV
Molecular Detection for Mycoplasma
Molecular detection of parvovirus B19 and Mycoplasma 

Selected Publications  

Fontes AM, Kashima S, Bonfim-Silva R, Azevedo R, Abraham KJ, Albuquerque SR, Bordin JO, Júnior DM, Covas DT. Association between Knops blood group polymorphisms and susceptibility to malaria in an endemic area of the Brazilian Amazon. Genet Mol Biol. 2011 Oct; 34(4): 539-45.

Haddad R, Kashima S, Rodrigues ES, Palma PV, Zago MA, Covas DT. Inhibition of expression of HTLV-1 structural genes mediated by short hairpin RNA in vitro. Anticancer Res. 2011 Jun; 31(6): 2173-7.

Slavov SN, Kashima S, Pinto AC, Covas DT. Human parvovirus B19: general considerations and impact on patients with sickle-cell disease and thalassemia and on blood transfusions. FEMS Immunol Med Microbiol. 2011 Aug; 62(3):247-62. doi: 10.1111/j.1574-695X.2011.00819.x.

Haddad R, Kashima S, Rodrigues ES, Azevedo R, Palma PV, de Magalhães DA, Zago MA, Covas DT. Silencing of HTLV-1 gag and env genes by small interfering RNAs in HEK 293 cells. J Virol Methods. 2011 Apr; 173(1): 92-8.

Tognon R, Gasparotto EP, Leroy JM, Oliveira GL, Neves RP, Carrara Rde C, Kashima S, Covas DT, Santana M, Souto EX, Zanichelli MA, Velano CE, Simões BP, Alberto FL, Miyashiro K, de Souza AM, Amarante-Mendes GP, de Castro FA. Differential expression of apoptosis-related genes from death receptor pathway in chronic myeloproliferative diseases. J Clin Pathol. 2011 Jan; 64(1):75-82.

Haddad R, Cilião Alves DC, Rocha-Junior MC, Azevedo R, do Socorro Pombo-de-Oliveira M, Takayanagui OM, Donadi EA, Covas DT, Kashima S. HLA-G 14-bp insertion/deletion polymorphism is a risk factor for HTLV-1 infection. AIDS Res Hum Retroviruses. 2011 Mar; 27(3):283-8.

Prata Kde L, Orellana MD, De Santis GC, Kashima S, Fontes AM, Carrara Rde C, Palma PV, Neder L, Covas DT. Effects of high-dose chemotherapy on bone marrow multipotent mesenchymal stromal cells isolated from lymphoma patients. Exp Hematol. 2010 Apr; 38(4):292-300.e4.

Sorgi CA, Secatto A, Fontanari C, Turato WM, Belangér C, de Medeiros AI, Kashima S, Marleau S, Covas DT, Bozza PT, Faccioli LH. Histoplasma capsulatum cell wall {beta}-glucan induces lipid body formation through CD18, TLR2, and dectin-1 receptors: correlation with leukotriene B4 generation and role in HIV-1 infection. J Immunol. 2009 Apr 1; 182(7): 4025-35.

Kashima S, Rodrigues ES, Azevedo R, da Cruz Castelli E, Mendes-Junior CT, Yoshioka FK, da Silva IT, Takayanagui OM, Covas DT. DC-SIGN (CD209) gene promoter polymorphisms in a Brazilian population and their association with human T-cell lymphotropic virus type 1 infection. J Gen Virol. 2009 Apr; 90(Pt 4):927-34.

Carrara RC, Orellana MD, Fontes AM, Palma PV, Kashima S, Mendes MR, Coutinho MA, Voltarelli JC, Covas DT. Mesenchymal stem cells from patients with chronic myeloid leukemia do not express BCR-ABL and have absence of chimerism after allogeneic bone marrow transplant. Braz J Med Biol Res. 2007 Jan; 40(1):57-67.