Silvia Regina Caminada de Toledo
Affiliated Professor and Laboratory Coordinator Pediatric Oncology Institute GRAACC/UNIFESP
silviatoledo@graacc.org.br
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Education  

1983-1986 Graduated in Biologic Sciences, Presbiterian Mackenzie University, Brazil
1987-1988 Improvement- Federal University of São Paulo
1989-1991 Master Thesis in Genetics, Federal University of São Paulo
1999 Research Student, Ontario Cancer Institute, University of Toronto, Canada
2000-2002 PhD in Science, Federal University of São Paulo 

Research Interests  

Cancer Genetics
Tumor Markers
Children Cancer 

Research Overview   Our group has sought to identify and understand the mechanisms of biological markers that may be involved in the diagnosis, prognosis and in treatment orientation of childhood and adolescence tumors. We concentrate our research in those tumors that still represent a major challenge in pediatric oncology, bone/soft tissue sarcomas and brain tumors. An important group of patients with these tumors are resistant to conventional therapies and another group has variable degrees of toxicity. Our main focus is to understand the genome of osteosarcoma host and the relationship between osteosarcoma cancer stem cell and drug resistance and response to treatment. 
Lab Staff  

PhD Students
Francine Tesser Gamba/ Scholarship- FAPESP
Indhira Dias Oliveira
Carolina Salinas de Souza/ Scholarship- FAPESP

Master Students
Robson Ramos de Souza/ Scholarship- CNPq
Bruna Mascaro Cordeiro/ Scholarship- CNPq
Gabriela Rampazzo Cruz/ Scholarship- CNPq

Technicians
Juliana Thomazini Gouveia (Molecular Genetics)
Michele Gaboardi de Carvalho (Cytogenetics)

Collaborators  

Wilson A Silva Junior, PhD
Associated Professor of Genetics, FMRP/USP
wilsonjr@usp.br 

International Collaborators  

Sharon A. Savage, MD, FAAP
Genome-Wide Association Study of Osteogenic Sarcoma
Investigator, Clinical Genetics Branch
Division of Cancer Epidemiology and Genetics, National Cancer Institute, NIH 6120 Executive Blvd., EPS/7018, Rockville, MD 20852
savagesh@mail.nih.gov

Sylvain Baruchel
Director of New Agent and Innovative Therapy Program
Professor –Pediatrics University of Toronto 
sylvain.baruchel@sickkids.ca 

Facilities Coordination  

DNA sequencing (ABI 3500 Genetic Analyzer)
7500 Real Time PCR System
7500 Fast Real Time PCR System
Ikaros/Isis Work Station –K24-CGH for BX51 

Selected Publications  

1. MAPK7 and MAP2K4 as prognostic markers in osteosarcoma. Tesser-Gamba F, Petrilli AS, de Seixas Alves MT, Filho RJ, Juliano Y, Toledo SR. Hum Pathol. 2011 Dec 8. [Epub ahead of print] PMID: 22154052 [PubMed – as supplied by publisher]

2. C-kit expression in human osteosarcoma and in vitro assays. Miiji LN, Petrilli AS, Di Cesare S, Odashiro AN, Burnier MN Jr, de Toledo SR, Garcia RJ, Alves MT. Int J Clin Exp Pathol. 2011;4(8):775-81 Epub 2011 Nov 3.

3. Expression analysis of stem cell-related genes reveal OCT4 as a predictor of poor clinical outcome in medulloblastoma. Rodini CO, Suzuki DE, Saba-Silva N, Cappellano A, de Souza JE, Cavalheiro S, Toledo SRC, Okamoto OK.J Neurooncol. 2011 Jul 2. [Epub ahead of print]

4. Insights on PRAME and osteosarcoma by means of gene expression profiling.Toledo SRC, Zago MA, Oliveira ID, Proto-Siqueira R, Okamoto OK, Severino P, Vêncio RZ, Gamba FT, Silva WA, Moreira-Filho CA, Torre CA, Alves MT, Garcia-Filho RJ, Simpson AJ, Petrilli AS. J Orthop Sci. 2011 Jul;16(4):458-66. Epub 2011 Jun 21.PMID:21691740

5. ASPM gene expression in medulloblastoma.Vulcani-Freitas TM, Saba-Silva N, Cappellano A, Cavalheiro S, Marie SK, Oba-Shinjo SM, Malheiros SM, de Toledo SRC.Childs Nerv Syst. 2011 Jan;27(1):71-4. Epub 2010 Aug 6.PMID:20694558

6. Low mRNA expression of the apoptosis-related genes CASP3, CASP8, and FAS is associated with low induction treatment response in childhood acute lymphoblastic leukemia (ALL). Mata JF, Silveira VS, Mateo EC, Cortez MA, Queiroz RG, Yunes JA, Lee ML, Toledo SRC, Petrilli AS, Brandalise SR, Tone LG, Scrideli CA. Pediatr Blood Cancer. 2010 Jul 15;55(1):100-7.PMID:20232432

7. Glutathione S-transferase polymorphisms in osteosarcoma patients.Salinas-Souza C, Petrilli AS, Toledo SRC.Pharmacogenet Genomics. 2010 Aug;20(8):507-15.PMID:20577141

8. Bone deposition, bone resorption, and osteosarcoma.Toledo SRC, Oliveira ID, Okamoto OK, Zago MA, de Seixas Alves MT, Filho RJ, Macedo CR, Petrilli AS. J Orthop Res. 2010 Sep;28(9):1142-8.PMID:20225287

9. Aberrant signaling pathways in medulloblastomas: a stem cell connection.Rodini CO, Suzuki DE, Nakahata AM, Pereira MC, Janjoppi L, Toledo SRC, Okamoto OK. Arq Neuropsiquiatr. 2010 Dec;68(6):947-52.PMID:21243257

10. mRNA expression of matrix metalloproteinases (MMPs) 2 and 9 and tissue inhibitor of matrix metalloproteinases (TIMPs) 1 and 2 in childhood acute lymphoblastic leukemia: potential role of TIMP1 as an adverse prognostic factor. Scrideli CA, Cortez MA, Yunes JA, Queiróz RG, Valera ET, da Mata JF, Toledo SRC, Pavoni-Ferreira P, Lee ML, Petrilli AS, Brandalise SR, Tone LG. Leuk Res. 2010 Jan;34(1):32-7. Epub 2009 Oct 28.PMID:19875168

11. mRNA expression profile of multidrug resistance genes in childhood acute lymphoblastic leukemia. Low expression levels associated with a higher risk of toxic death.Cortez MA, Scrideli CA, Yunes JA, Valera ET, Toledo SRC, Pavoni-Ferreira PC, Lee ML, Petrilli AS, Brandalise SR, Tone LG.Pediatr Blood Cancer. 2009 Dec;53(6):996-1004.PMID:19672972

12. A simplified minimal residual disease polymerase chain reaction method at early treatment points can stratify children with acute lymphoblastic leukemia into good and poor outcome groups.Scrideli CA, Assumpção JG, Ganazza MA, Araújo M, Toledo SRC, Lee ML, Delbuono E, Petrilli AS, Queiróz RP, Biondi A, Viana MB, Yunes JA, Brandalise SR, Tone LG.Haematologica. 2009 Jun;94(6):781-9.PMID:19483156

13. Association of EGFR c.2073A>T polymorphism with decreased risk of diffusely infiltrating astrocytoma in a Brazilian case-control study. Barbosa KC, Oba-Shinjo SM, Uno M, Carvalho PO, Rosemberg S, Aguiar PH, Carlotti CG, Malheiros SM, Toledo SRC, Lotufo P, Marie SK.Int J Biol Markers. 2008 Jul-Sep;23(3):140-6.PMID:18949739

14. Maternal embryonic leucine zipper kinase transcript abundance correlates with malignancy grade in human astrocytomas.Marie SK, Okamoto OK, Uno M, Hasegawa AP, Oba-Shinjo SM, Cohen T, Camargo AA, Kosoy A, Carlotti CG Jr, Toledo SRC, Moreira-Filho CA, Zago MA, Simpson AJ, Caballero OL.Int J Cancer. 2008 Feb 15;122(4):807-15.PMID:17960622

15. TNF-alpha, TNF-beta, IL-6, IL-10, PECAM-1 and the MPO inflammatory gene polymorphisms in osteosarcoma.Oliveira ID, Petrilli AS, Tavela MH, Zago MA, de Toledo SRC. J Pediatr Hematol Oncol. 2007 May;29(5):293-7.PMID:17483704

16. Expression of major vault protein gene in osteosarcoma patients. Dalla-Torre CA, de Toledo SRC, Yoshimoto M, Petrilli AS, Andrade JA, Chilton-MacNeill S, Squire JA, Zielenska M.J Orthop Res. 2007 Jul;25(7):958-63.PMID:17415755

17. Effects of THBS3, SPARC and SPP1 expression on biological behavior and survival in patients with osteosarcoma.Dalla-Torre CA, Yoshimoto M, Lee CH, Joshua AM, de Toledo SRC, Petrilli AS, Andrade JA, Chilton-MacNeill S, Zielenska M, Squire JA.BMC Cancer. 2006 Oct 5;6:237.PMID:17022822

18. Metaphase and array comparative genomic hybridization: unique copy number changes and gene amplification of medulloblastomas in South America.Yoshimoto M, Bayani J, Nuin PA, Silva NS, Cavalheiro S, Stavale JN, Andrade JA, Zielenska M, Squire JA, de Toledo SRC.Cancer Genet Cytogenet. 2006 Oct 1;170(1):40-7.PMID:16965953

19. Comparative genomic hybridization analysis of pediatric adamantinomatous craniopharyngiomas and a review of the literature.Yoshimoto M, de Toledo SRC, da Silva NS, Bayani J, Bertozzi AP, Stavale JN, Cavalheiro S, Andrade JA, Zielenska M, Squire JA.J Neurosurg. 2004 Aug;101(1 Suppl):85-90. Review.PMID:16206977