Center for Cell-based Therapy – Page 79 – Centro de Terapia Celular

Lygia da Veiga Pereira

		Lygia V. Pereira Full Professor of Genetics, IB-USP Head of the National Laboratory of Embryonic Stem Cells (LaNCE) lpereira@usp.br PubMed
Education		1984-1989 Bachelor in Physiscs, Pontifícia Universidade Católica, Rio de Janeiro, RJ. 1989-1990 M.S. in Molecular Biology, Instituto de Biofísica Carlos Chagas Filho, UFRJ, Rio de Janeiro, RJ. 1989-1994 PhD in Human Molecular Genetics, City University of New York, Mount Sinai Graduate School, New York, NY, USA. 2004 Full Professor, University of São Paulo, Brazil.
Research Interests		Human Molecular Genetics Marfan Syndrome Pluripotent Stem Cells Epigenetics – X chromosome inactivation
Research Overview		Major Research Lines: (1) Marfan Syndrome (MFS) – lessons from animal models: MFS is an autosomal dominant disease of the connective tissue that affects the ocular, skeletal and cardiovascular systems. The disease is caused by mutations in the FBN1 gene, that code the fibrillin-1 protein, a major structural component of the extracellular microfibers. By homologous recombination in ES cells, we have established two mouse models for MFS: one containing a FBN1 null allele (mgNull), and another with a deletion of exons 18-25 (mgΔloxPneo). In these models we study the mechanisms of pathogenesis of the disease; genetic and epigenetic factors modifying the phenotype, and we try new therapies for MFS. (2) Epigenetic inheritance – X chromosome inactivation (XCI): we are particularly interested in the process of maintenance of the inactive state of the X chromosome throughout mitosis in human cells. For that, we have developed functional tests that allow the screening of new genes involved in this process. We also work in the characterization of human embryonic stem cells as an in vitro model for the study of XCI. (3) Establishment of new lines of human embryonic stem cells (hESC): Our group is involved in the derivation of new lines of hESC from the Brazilian population, for use in research and therapy. Our interest is to study the epigenetic state of these cells cultured/derived in different conditions. (4) Analysis of the role of GBA mutations in the development of Parkinsonism using induced pluripotent stem cells (iPSC): Recent works have shown a higher frequency of Gaucher disease causing GBA mutations in patients with Parkinsonism (PD). This projects aims the establishment of iPSC lines from patients with PD and mutations in the GBA gene, where we will be able to study the molecular mechanisms behind this association. (5) National Laboratory of Emrbyonic Stem Cells (LaNCE): this is one of eight Centers for Cell Technology sponsored by the Minsitries of Health, and Science and Technology for the production of stem cells under GMP conditions. LaNCE’s specific mission is to promote research and therapy with pluripotetn stem cells in Brazil. This is a project in collaboration with Prof. Stevens Rehen’s group, at UFRJ.
Lab Staff		Molecular Genetics Group Erica Sara M.S. , Student/FAPESP Fernando Sábio M.S., Student/FAPESP Gustavo Ribeiro Fernandes, PhD Student/FAPESP Fernanda Silvestre, PhD Student/CAPES Ana Maria Fraga, PhD Student/FAPESP Cynthia Quinderé Cardoso, PhD Student/CNPq Joana Carvalho Moreira de Mello, PhD Student/FAPESP Naja Vergani, PhD Student/CAPES Giovana Pirolla Cardozo, PhD Student/FAPESP Simone Fonseca, Post-Doc/CAPES Bruno Lazzari Lima, Post-Doc/FAPESP Mariana Morato Marques, Technical support Ana Patrícia Kamisaki Santana, Administrative support
Collaborators		Egberto Reis Barbosa, PhD Universidade de São Paulo, Faculdade de Medicina da Universidade de São Paulo, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo. Av. Dr. Enéas de Carvalho Aguiar, 255 Cerqueira César 05403-000 – Sao Paulo, SP – Brasil Telefone: (11) 30696401 Fax: (11) 30827548 http://www.fm.usp.br/ Silvia Maria Gomes Massironi, PhD Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Imunologia. AV. LINEU PRESTES 1730 CIDADE UNIVERSITARIA 05508-900 – Sao Paulo, SP – Brasil Telefone: (11) 30917398 Fax: (11) 30917224
International Collaborators		Jeanne Loring, PhD Professor of Developmental Neurobiology Director of the Center for Regenerative Medicine Department of Chemical Physiology California Campus Laboratory Website jloring@scripps.edu (858) 784-7767
Facilities Coordination		GMP facility for cell culture
Selected Publications		FRAGA AM, ARAÚJO ESS, STABELLINI R, VERGANI N, PEREIRA LV. A Survey of Parameters Involved in the Establishment of New Lines of Human Embryonic Stem Cells. Stem Cell Reviews, v.1, p.1 – , 2011.AMPS K, ANDREWS PW, ANYFANTIS G, ARMSTRONG L, AVERY S, BAHARVAND H, BAKER J, BAKER D, MUNOZ MB, BEIL S, BENVENISTY N, BEN-YOSEF D, BIANCOTTI JC, BOSMAN A, BRENA RM, BRISON D, CAISANDER G, CAMARASA MV, CHEN J, CHIAO E, CHOI YM, CHOO ABH, COLLINS D, COLMAN A, CROOK J M, DALEY GQ, DALTON A, DE SOUSA PA, DENNING C, DOWNIE J, DVORAK, MONTGOMERY KD, FEKI A, FORD A, FOX V, FRAGA AM, FRUMKIN T, GE L, GOKHALE PJ, GOLAN-LEV T, PEREIRA, LV. et al. Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. Nature Biotechnology, 29:1132–1144, 2011.Fraga, A.M. ; Soukoyan, M. ; Rajan, P ; de Almeida Ferreira Braga, Daniela ; IACONELLI JR., A. ; José Gonçalves Franco Jr ; BORGES JR., E. ; Pereira, L.V. . Establishment Of A Brazilian Line Of Human Embryonic Stem Cells In Defined Medium – Implications For Cell Therapy In An Ethnically Diverse Population. Cell Transplantation, v. 1, p. 1, 2010.Moreira de Mello, Joana Carvalho ; Araújo, Érica Sara Souza de ; Stabellini, Raquel ; Fraga, Ana Maria ; Souza, Jorge Estefano Santana de ; Sumita, Denilce R. ; Camargo, Anamaria A. ; Pereira, Lygia V. . Random X Inactivation and Extensive Mosaicism in Human Placenta Revealed by Analysis of Allele-Specific Gene Expression along the X Chromosome. Plos One, v. 5, p. e10947, 2010.E. Sidransky, et al. Multicenter Analysis of Glucocerebrosidase Mutations in Parkinson’s Disease. New England Journal of Medicine, 361:1651-61, 2009.Raquel Stabellini, Joana Carvalho Moreira de Mello, Lys Molina Hernandes, Lygia V. Pereira. MAOA and GYG2 are submitted to X chromosome inactivation in human fibroblasts. Epigenetics, 4:1-8, 2009.VASQUES, L.R., STABELINI, R., SUKOYAN, M., E PEREIRA, L.V. XIST repression in the absence of DNMT1 and DNMT3B. DNA Research, 12:373-378, 2005.CARTA C, PEREIRA L, ARTEAGA-SOLIS E, LEE-ARTEAGA SY, STARCHER B, MERKEL CA, SUKOYAN M, KERKIS A, HAZEKI N, KEENE DR, SAKAI LY E RAMIREZ F. Fibrillins 1 and 2 perform partially overlapping roles during aortic development. Journal of Biological Chemistry 281(12):8016-8023, 2005.XUE, F., TIAN, C., DU, F., KUBOTA, C., TANEJA, M., DINNYES, A., DAI, Y., LEVINE, H., PEREIRA, L.V., E YANG, X. Aberrant patterns of X chromosome inactivation in bovine clones. Nature Genetics, vol. 31(6):216-220, 2002.Sukoyan, M.A., Kerkis, A.Y., Mello, M.R.B., Kerkis, I.E., Visintin, J.A., Pereira, L.V. Establishment of new murine embryonic stem (ES) cell lines for the generation of mouse models for human genetic diseases. Braz. J. Med. Biol. Res., vol. 35(5):535-542, 2002.Vasques, L.R., and Pereira, L.V. Allele-specific X-linked gene activity in normal human cells assayed by expressed single nucleotide polymorphisms (cSNPs). DNA Research, vol. 8(4):173-177, 2001.

Lewis Joel Greene

		Lewis Joel Greene Full Professor of Cell and Molecular Biology (retired) FMRP/USP Supervisor of the Center for Protein Chemistry, at Regional Blood Center of Ribeirão Preto Volunteer head teacher (senior associate) of FMRP/USP ljgreene@fmrp.usp.br PubMed Scopus ResearchID Scielo Scholar
Education		1951-1955 B.A (magna cum laude), Amherst College, Amherst Massachusetts 1955-1962 Ph D, Rockefeller University, C.H.W. HIRS and G.E. Palade (Research advisors) 1990 Full Professor, University of São Paulo, Brazil
Research Interests		Structure and function of proteins Protein- protein interactions Proteomics as an entry to cell biology Molecular basis of cancer Molecular basis of sepsis
Research Overview		The recent focus of our group has been to understand the molecular mechanisms involved in the development of diseases and cancer . We are collaborating with biologists, clinicians and chemists using the methodologies of protein chemistry and molecular biology to identify biomarkers for diagnosis and prognosis and to understand the processes involved.
Lab Staff		José César Rosa, PhD – Laboratory Collaborator
Collaborators		Carolina Hassibe Thomé, Pos Doc Germano Aguiar Ferreira, Doctoral Student José César Rosa Faculdade de Medicina de Ribeirão Preto – Universidade de São Paulo Centro de Química de Proteínas, Hemocentro de Ribeirão Preto, SP jcrosa@fmrp.usp.br Andréia Machado Leopoldino Departamento de Análises Clínicas, Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo andreiaml@usp.br Dimas Tadeu Covas Faculdade de Medicina de Ribeirão Preto – Universidade de São Paulo Centro Regional de Hemoterapia de Ribeirão Preto dimas@fmrp.usp.br Vitor Marcel Faça Prof. Dr. da Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo Department of Biochemistry and Immunology vitor.faca@fmrp.usp.br Eduardo Magalhães Rego Departamento de Clínica Médica da Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo edumrego@hotmail.com Wilson Araújo da Silva Júnior Departamento de Genética da Faculdade de Medicina de Ribeirão Preto – Universidade de São Paulo wilsonjr@usp.br Roger Chammas Professor Titular, Faculdade de Medicina, Universidade de São Paulo – USP Departamento de Radiologia rchammas@lim24.fm.usp.br
International Collaborators
Facilities Coordination		Mass Spectrometry and Protein Chemistry Lab
Selected Publications		1. SOBRAL, L. M. ; SOUSA, L.O. ; COLETTA, R. D. ; CABRAL, H. ; GREENE, L. J. ; TAJARA, E.H. ; GUTKIND, J. S. ; CURTI, C. ; Leopoldino, A. M. . Stable SET knockdown in head and neck squamous cell carcinoma promotes cell invasion and the mesenchymal-like phenotype in vitro, as well as necrosis, cisplatin sensitivity and lymph node metastasis in xenograft tumor models. Molecular Cancer , v. 13, p. 32, 2014. 2. Thomé, C. H. ; DOS SANTOS, G. A. ; FERREIRA, G. A. ; SCHEUCHER, P. S. ; IZUMI, C. ; Leopoldino, A. M. ; SIMAO, A. M. ; CIANCAGLINI, P. ; DE OLIVEIRA, K. T. ; CHIN, A. ; HANASH, S. M. ; FALCAO, R. P. ; REGO, E. M. ; Greene, L. J. ; Faça, V. M. . Linker for Activation of T-cell Family Member2 (LAT2) a Lipid Raft Adaptor Protein for AKT Signaling, Is an Early Mediator of Alkylphospholipid Anti-leukemic Activity. Molecular & Cellular Proteomics , v. 11, p. 1898-1912, 2012. Citações: 3\| 4 3. Fontes, A. M. ; Melo, F.U.F. ; Greene, L.J. ; Faça, V. M. ; Lin, Y. ; Gerson, S.L. ; Covas, D. T. . Production of human factor VIII-FL in 293T cells using the bicistronic MGMT(P140K)-retroviral vector. Genetics and Molecular Research , v. 11, p. 775-789, 2012. 4. Miranda, H. C. ; HERAI, R. H. ; Thomé, C. H. ; Gomes, G. G. ; PANEPUCCI, R. A. ; ORELLANA, M. D. ; Covas, D. T. ; Muotri, A. R. ; Greene, L.J. ; Faça, V. M. . A quantitative proteomic and transcriptomic comparison of human mesenchymal stem cells from bone marrow and umbilical cord vein. Proteomics (Weinheim. Print) , v. 12, p. 2607-2617, 2012. Citações: 3\| 6 5. Spiller, F. ; FERREIRA, S. H. ; Greene, L. J. ; Rosa, J. C. ; Altruda, F. ; Souto, F. O. ; Hirsch, E. ; Mestriner, F. L. A. C. ; Cunha, F. Q. ; Tolosano, E. ; Alves-Filho, J. C. ; Laure, H. J. ; Freitas, A. ; Costa, C. ; Vinchi, F. . Inhibition of Neutrophil Migration by Hemopexin Leads to Increased Mortality Due to Sepsis in Mice. American Journal of Respiratory and Critical Care Medicine , v. 183, p. 922-931, 2011. Citações: 7\| 7 6. Santos, G. A. S. ; Thomé, C. H. ; FERREIRA, G.A. ; YONEDA, J. S. ; NOBRE, T. M. ; Daghastnali, K.R.P. ; SCHEUCHER, P. S. ; TEIXEIRA, H. L. ; Constantino, M. G. ; DE OLIVEIRA, K. T. ; Faça, V. M. ; FALCAO, R. P. ; GREENE, L. J. ; REGO, E. M. ; CIANCAGLINI, P. . Interaction of 10-(octyloxy) decyl-2-(trimethylammonium) ethyl phosphate with mimetic membranes and cytotoxic effect on leukemic cells. Biochimica et Biophysica Acta. Biomembranes , v. 1798, p. 1714-1723, 2010. Citações: 4\| 5 7. Mestriner, F. L. A. C. ; Spiller, F. ; Laure, H. J. ; Souto, F. O. ; TAVARES-MURTA, B.M. ; Rosa, J. C. ; Basile Filho, A. ; FERREIRA, S. H. ; GREENE, L. J. ; Cunha, F. Q. . Acute-phase protein α -1-acid glycoprotein mediates neutrophil migration failure in sepsis by a nitric oxide-dependent mechanism.. Proceedings of the National Academy of Sciences of the United States of America (Online) , v. 104, p. 19595-19600, 2007. Citações: 20\| 22 8. SOUZA, G. A. ; GODOY, L.M.F. de ; TEIXEIRA, V. R. ; OTAKE, A. H. ; SABINO, A. ; Rosa, J. C. ; Dinarte, A. R. ; Pinheiro, D. G. ; Silva Jr, W. A. ; EBERLIN, M. N. ; Chammas, R. ;GREENE, L. J. . Proteomic and SAGE profiling of murine melanoma progression indicates the reduction of proteins responsible for ROS degradation. Proteomics, Inglaterra, v. 6, n.5, p. 1460-1470, 2006. Citações: 22\| 22

Flávio Vieira Meirelles

		Flávio V. Meirelles Full Professor of Cell Biology & Development, FZEA/USP Member of the Coordinating Committee (Veterinary Medicine) of FAPESP Associate editor of Genetics And Molecular Research meirellf@usp.br PubMed Scopus ORCID ResearcherID Scholar
Education		1989-1993 Graduated in Veterinary Medicine, São Paulo State University, Brazil 1994-1996 Master Thesis in Reproduction, University of Montreal, Canada 1997-1999 PhD in Genetics, Medical Scholl of Ribeirão Preto, University of São Paulo 2004 Full Professor, University of São Paulo, USP, Brazil
Research Interests		Cytoplasmic inheritance Nuclear Reprograming Animal Genetics Mitochondrial genome Embryonic genome activation
Research Overview		Since the last 20 years we have focused on understanding the process of cytoplasmic inheritance. We have successfully created animal models to study mtDNA inheritance, and its related cell death process during early embryo development, which allowed us to develop a patent on SCNT animal production. We also dedicated to understand the process of cytoplasmic inheritance and its effect on nuclear reprograming, especially on the imprinting modifications after in vitro manipulation such as cloning or reprograming somatic cells. Recently we developed an interesting method for depletion of specific organelles or compounds in oocytes, zygotes or cells that allowed us to test for their effect on embryogenesis and to introduce other organelles or compounds (we did it on mitochondria and we are now testing on lipid granules) in order to partially control their inheritance. Within the next 11 years, we will together with the CTC group continue to work on this area willing to evaluate the production of reprogrammed cells to autologous cell therapy and to control the cytoplasmic inheritance in order to apply to cell based therapy.
Lab Staff		Pos Docs Marcos Roberto Chiaratti, PhD Lilian de Jesus Oliveira,PhD José Rodrigo V. Pimentel, PhD PhD Fabiana Fernandes Bressan,MSc Reno Roldi Araujo, MSc Rodrigo Barreto, MSc Rafael Sampaio, MSc Juliano Sangalli, MSc Pedro Ratto Pires, MSc MSc Lais Vicari Pessoa, DVM Gabriella Mamede Andrade, DVM
Collaborators		Claudia Lima Verde Leal, PhD Universidade Federal de São Paulo LMMD Carlos Eduardo Ambrósio, PhD Universidade Federal de São Paulo LMMD Felipe Perecin, PhD Universidade Federal de São Paulo LMMD Heidge Fukumasu Universidade Federal de São Paulo LMMD Maria Angélica Miglino Universidade Federal de São Paulo FMVZ – Programa de Pós Graduação em Anatomia
International Collaborators		Joanna Poulton, PhD Professor of Genetics Univ. of Oxford, Lawrence Charles Smith, PhD Full Professor – Canadian Chair of Animal Cloning Université de Montréal Willian Allan King Full Professor – Canadian Chair of Animal Biotechnology Guelph – ON Canada
Facilities Coordination		Embryo Manipulation Facilities including IVF Laboratory, Cell sorting, Basic Molecular Biology lab. Animal Hospital Facility dedicated for Innovative Therapy.
Selected Publications		1. BRESSAN, Fabiana Fernandes ; Dos Santos Miranda, Moyses ; PERECIN, Felipe ; De Bem, Tiago Henrique ; Pereira, Flavia Thomaz Verechia ; Russo-Carbolante, Elisa Maria ; Alves, Daiani ; Strauss, Bryan ; Bajgelman, Marcio ; Krieger, José Eduardo ; Binelli, Mario ; Meirelles, Flavio Vieira . Improved Production of Genetically Modified Fetuses with Homogeneous Transgene Expression After Transgene Integration Site Analysis and Recloning in Cattle. Cellular Reprogramming (Formerly Cloning and Stem Cells), v.13 (1) p.29-36, 2011. 2. CHIARATTI, Marcos Roberto; FERREIRA, Christina Ramires; PERECIN, Felipe … Meirelles, Flávio Vieira . Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. Reprod. BioMedicine Online, v. 22, p. 172-183, 2011. 3. Suzuki Jr, João ; Therrien, Jacinthe ; Filion, France ; Lefebvre, Rejean …Perecin, Felipe ; Meirelles, Flávio V. ; Smith, Lawrence Charles . Loss of Methylation at H19 DMD Is Associated with Biallelic Expression and Reduced Development in Cattle Derived by Somatic Cell Nuclear Transfer. Biology of Reproduction, v. 84, p. 947-956, 2011. 4. Bressan, Fabiana Fernandes; Perecin, Felipe; Sangalli, Juliano Rodrigues ; Meirelles, F. V. Reprogramming somatic cells: pluripotency through gene induction and nuclear transfer. Acta Scientiae Veterinariae, v. 39, p. s83-s95, 2011. 5. De Bem, T. H. C. ; Chiaratti, M. R. ; Rochetti, R. ; Bressan, F. F. ; Sangalli, J. R. ; Miranda, M. S. ; Pires, P. R. L. ; Schwarz, K.R.L. ; Sampaio, R. V. ; Fantinato Neto, P. ; Pimentel, J. R. V. ; Perecin, F. ; Smith, L. C. ; Meirelles, F. V. ; Leal, C. L. V. . Viable calves produced by somatic nuclear transfer using meiotic-blocked oocytes. Cellular reprogramming, v. 13, p. DOI: 10.1089/c, 2011. 6. Chiaratti, M R ; Meirelles, F. V. ; Wells, D ; Poulton, J . Therapeutic treatments of mtDNA diseases at the earliest stages of human development. Mitochondrion (Amsterdam. Print), v. 11, p. 820-828, 2011. 7. Ferreira, C. R. ; Burgstaller, J. P. ; Perecin, F. ; Garcia, J. M. ; Chiaratti, M. R. ; Meo, S. C. ; Muller, M. ; Smith, L. C. ; Meirelles, F. V. Pronounced Segregation of Donor Mitochondria Introduced by Bovine Ooplasmic Transfer to the Female Germ-Line. Biology of Reproduction, v. 82, p. 563-571, 2010. 8. Meirelles, Flávio V. ; Birgel, Eduardo H. ; PERECIN, Felipe Et Al., Delivery of cloned offspring: experience in Zebu cattle. Reprod., Fertility and Dev. v. 22, p. 88, 2010. 9. Chiaratti, M. R. ; Meirelles, F. V. . Mitochondrial DNA Copy Number, a Marker of Viability for Oocytes. Biology of Reproduction, v. 83, p. 1-2, 2010. 10. Ferreira, Christina Ramires ; Saraiva, Sérgio Adriano ; Catharino, Rodrigo Ramos ; Garcia, Jerusa Simone ; Gozzo, Fábio César ; Sanvido, Gustavo Braga ; Santos, Luiz Fernando Arruda ; Turco, Edson Guimarães Lo ; Pontes, José Henrique Fortes ; Basso, Andréa Cristina ; Bertolla, Ricardo Pimenta ; Sartori, Roberto ; Guardieirio, Monique Mendes ; Perecin, Felipe ; Meirelles, F. V; Eberlin, Marcos Nogueira . Single embryo and oocyte lipid fingerprinting by mass spectrometry. Journal of Lipid Research (Print), v. 51, p. 1218-1227, 2010. 11. Chiaratti, Marcos Roberto ; Ferreira, Christina Ramires ; Meirelles, Flávio Vieira ; et al. Xenooplasmic Transfer between Buffalo and Bovine Enables Development of Homoplasmic Offspring. Cellular Reprogramming (Formerly “Cloning and Stem Cells”), v. 12, p. 231-236, 2010. 14. Poulton, Joanna ; Chiaratti, Marcos R. ; Meirelles, Flávio V. ; Kennedy, Stephen ; Wells, Dagan ; Holt, Ian J. ; Suomalainen, Anu . Transmission of Mitochondrial DNA Diseases and Ways to Prevent Them. PLoS Genetics, v. 6, p. e1001066-08, 2010. 15. Perecin, F. ; Meo, S.C. ; Yamazaki, W. ; Ferreira, C.R. ; Merighe, G.K.F. ; Meirelles, F.V. ; Garcia, J.M. . Imprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes. Gen. and Mol. Res., v. 8, p. 76-85, 2009. 16. Dos Santos Miranda, Moysés ; BRESSAN, Fabiana Fernandes ; Zecchin, Karina Gottardello ; Vercesi, Anibal Eugênio ; MESQUITA, Ligia Garcia ; Fonseca Merighe, Giovana Krempel ; KING, William Allan ; Ohashi, Otávio Mitio ; Pimentel, José Rodrigo Valim ; PERECIN, Felipe ; Meirelles, Flávio Vieira . Serum-Starved Apoptotic Fibroblasts Reduce Blastocyst Production but Enable Development to Term after SCNT in Cattle. Cloning and Stem Cells, v. 11, p. 1-9, 2009. 17 Chiaratti, M. R. ; Bressan, F. F. ; Ferreira, C. R. ; Caetano, A. R. ; Smith, L. C. ; Vercesi, A. E. ; Meirelles, F. V. . Embryo Mitochondrial DNA Depletion Is Reversed During Early Embryogenesis in Cattle. Biology of Reproduction, v. 82, p. 76-85, 2009. 18. Ferreira, E.M. ; Vireque, A.A. ; Adona, P.R. ; Meirelles, F.V. et al., Cytoplasmic maturation of bovine oocytes: Structural and biochemical modifications and acquisition of developmental competence. Theriogenology, v. 71, p. 836-848, 2009. 19. BIASE, Fernndo Henrique ; MARTELLI, L ; MERIGHE, Giovana Krempel Fonseca ; BIASE, Weruska Karyna Freitas Santos ; MIRANDA, Moysés Dos Santos ; SMITH, L. C. ; Meirelles, F. V.A retrospective model of oocyte competence: global mRNA and housekeeping transcripts are not associated with in vitro developmental outcome.. Zygote (Cambridge), v. 17, p. 289-295, 2009. 20. Bressan, F.F. ; De Bem, T.H.C. ; Perecin, F. ; Lopes, F.L. ; Ambrosio, C.E. ; Meirelles, F.V. ; Miglino, M.A. . Unearthing the Roles of Imprinted Genes in the Placenta. Placenta (Eastbourne), v. 30, p. 823-834, 2009.

Eduardo Magalhães Rego

		Eduardo Magalhães Rego Professor of Hematology/Oncology, Medical School of Ribeirão Preto, University of São Paulo Head of the Hematology and Clinical Oncology Service of the University Hospital (HC-FMRP) Coordinator in Brazil of the International Consortium on Acute Leukemias which is sponsored by the American Society of Hematology Member of the Board of Directors of the Brazilian Association of Hematology (ABHH) Editor of the Brazilian Journal of Medical and Biological Research and Associate Editor of Blood and Annals of Hematology emrego@hcrp.fmrp.usp.br PubMed Scopus ORCID ResearcherID Scholar
Education		1983-1988 Doctor of Medicine (M.D.) Medical School of Ribeirão Preto of the University of São Paulo 1989-1992 Medical Residence/ Fellowship in Hematology, University Hospital, Medical School of Ribeirão Preto of the University of São Paulo 1993-1997 Doctor of Philosophy (Ph.D.) Medical School of Ribeirão Preto of the University of São Paulo 1998-2001 Post-Doctoral Fellowship Memorial Sloan-Kettering Cancer Center, New York, USA 2002 Full Professor, University of São Paulo, USP, Brazil 2005 PhD in Medicine, Fox Chase Cancer Center, FCCC, USA
Research Interests		Hematological malignancies, in particular acute leukemias Leukemogenesis Animal models of acute leukemia Leukemia and cancer stem cells BV-CDI FAPESP
Research Overview		The main focus of our group is to integrate basic and clinical research on acute leukemias, in special acute myeloid leukemias. In order to have a better insight on the molecular basis of leukemogenesis, we have been studying acute promyelocytic leukemia (APL) as a model. We have been addressing the following questions: 1. Which cell subpopulation acts the leukemia initiating cells and how is quiescence and energy generation regulated in this subset; 2. Which microRNAs act as key regulators of differentiation in APL blasts and whether their expression profile are associated with treatment outcome; 3. Does the expression of transcriptinally active and inactive isoforms of the p73 gene affect the response to ATRA and if so how? 4. What is the role of microparticles and annexin II expression in APL-associated coagulopathy; 5. Can the molecular monitoring of the PML/RARA fusion gene using RQ-PCR technology predict relapse better than the routinely used RT-PCR method?; 6. Is leukemia relapse is associated with additional mutagenic events? 7. In leukemia prone syndromes, such as dyskeratosis congenita, which pathways confer survival advantage to hematopoietic progenitors and are associated in malignant transformation. Finally, our group is coordinating in Brazil the International Consortium on APL, which is a trial aiming to improve the treatment outcome of APL patients in developing countries.
Lab Staff		1. PhD students: a. Ana Paula A. de L. Lange b. Antonio R. L. de Araújo c. Helder Henrique Paiva d. Sarah Cristina Bassi 2. MsC students: a. Katarina Holanda 3. Post-docs: a. Priscila Santos Scheucher b. Guilherme Augusto Silva dos Santos Molecular Biology Technicians Ana Sílvia Gouveia Lima
Collaborators		Andréia M Leopoldino, PhD Depto de Análises Clínicas, Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo
International Collaborators		Dr Davide Rugero, Associate Professor Helen Diller Family Cancer Research Center University of California, San Francisco (UCSF) Mission Bay Campus 1450 3rd Street Room HD 386, MC 3110 San Francisco, CA 94158 Tel: 415/514-9755 Fax: 415/514-4826 davide.ruggero@ucsf.edu Prof. Ellen Solomon, Prof. David Grimwade, King’s College London School of Medicine Department of Medical and Molecular Medicine Floor 8 Tower Wing Guy’s Hospital London SE1 9RT Telephone : 0207 1882579 david.grimwade@genetics.kcl.ac.uk http://www.kcl.ac.uk/medicine/research/divisions/gmm/sections/clusters/cancer/solomon/index.aspx http://www.kcl.ac.uk/medicine/research/divisions/gmm/sections/clusters/cancer/grimwade/pgrimwade.aspx Prof. Hau C.Kwaan, Marjorie C. Barnett Professor in Hematology-Oncology Professor of Medicine Northwestern University Feinberg School of Medicine Room 8258, Olson Pavilion 710 N.Fairbanks Court, Chicago, IL. 60611 h-kwaan@northwestern.edu http://fsmweb.northwestern.edu/faculty/FacultyProfile.cfm?xid=14961 Prof. Stefan Bohlander, Department of Medicine III, Universität München, 81377 Munich, Germany bohlander@helmholtz-muenchen.de http://www.sfb684.med.uni-muenchen.de/projects/a6bohlander/index.html
Facilities Coordination		Flow Cytometry and Sorting Facility, And Small Animals Irradiator Facility, Medical School of Ribeirão Preto, University of São Paulo, FAPESP EMU – grant 09/54218-1
Selected Publications		Benicio MTL et al. Evaluation of the European LeukemiaNet recommendations for predicting outcomes of patients with acute myeloid leukemia treated in low- and middle-income countries (LMIC): A Brazilian experience. Leuk Res. 2017; 60:109-114.. Corrêa de Araujo Koury L et al. Treating acute promyelocytic leukaemia in Latin America: lessons from the International Consortium on Acute Leukaemia experience. Br J Haematol. 2017;177(6):979-983. Bittencourt R et al. Guidelines on the treatment of acute myeloid leukemia: Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular: Project guidelines: Associação Médica Brasileira – 2015. Rev Bras Hematol Hemoter. 2016; 38(1):58-74. Mota JM et al. Post-Sepsis State Induces Tumor-Associated Macrophage Accumulation through CXCR4/CXCL12 and Favors Tumor Progression in Mice. Cancer Immunol Res. 2016;4(4):312-22. Lucena-Araujo AR et al. High ΔNp73/TAp73 ratio is associated with poor prognosis in acute promyelocytic leukemia. Blood. 2015; 12;126(20):2302-6. Sanz MA et al. All-trans retinoic acid with daunorubicin or idarubicin for risk-adapted treatment of acute promyelocytic leukaemia: a matched-pair analysis of the PETHEMA LPA-2005 and IC-APL studies. Ann Hematol. 2015; 94(8):1347-56. Rego EMet al. Improving acute promyelocytic leukemia (APL) outcome in developing countries through networking, results of the International Consortium on APL. Blood. 2013; 121(11):1935-43. dos Santos GA et al. (+)α-Tocopheryl succinate inhibits the mitochondrial respiratory chain complex I and is as effective as arsenic trioxide or ATRA against acute promyelocytic leukemia in vivo. Leukemia 2012;26(3):451-60. Yoon A et al. Impaired control of IRES-mediated translation in X-linked dyskeratosis congenita. Science. 2006;312(5775):902-6.10. Rego EM et al. Retinoic acid (RA) and As2O3 treatment in transgenic models of acute promyelocytic leukemia (APL) unravel the distinct nature of the leukemogenic process induced by the PML-RARalpha and PLZF-RARalpha oncoproteins. Proc Natl Acad Sci U S A. 2000;97(18):10173-8.