Dimas Tadeu Covas
Full Professor of Clinical Medicine – Hematology and Transfusion Medicine
Director-President of Regional Blood Center of Ribeirão Preto – FMRP-USP
Deputy Coordinator of the National Institute of Science and Technology in Stem Cells and Cell Therapy
President of the Brazilian Association of Hematology and Hemotherapy
Full Member of the Brazilian Technical Commission on Bio-security (CTNBio)
Full Member of the Technical Board of Hematology for Federal Council of Medicine (CFM)
dimas@fmrp.usp.br
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Education 1976-1981 Graduated in Medicine, Medical School of Ribeirão Preto, University of São Paulo, Brazil
1982 Rotating Resident in Clinical Medicine, University Hospital of Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil
1983-1984 Resident in Hematology/Hemotherapy, University Hospital of Medical School of Ribeirão Preto, University of São Paulo, Brazil
1984-1986 Master in Medicine, Medical School of Ribeirão Preto, University of São Paulo, Brazil
1986 -1993 PhD in Medicine, Medical School of Ribeirão Preto, University of São Paulo, Brazil
1999 Full Professor
Research Interests Somatic and Pluripotent Stem Cells
Cell and Gene Therapy
Cell Culture
Functional Genomics
Recombinant Protein Expression
Sickle Cell Anemia
Blood Transfusion
Virology
Research Overview

Our main lines of research are:
1. Mesenchymal Stem Cell – isolation, functional characterization, production on a large scale in bioreactors, biodistribution and clinical potential of isolated MSC of diverse tissues as feral adnexa, umbilical cord, vascular fraction of the adipose tissue and bone marrow. We are also developing clinical studies of phase I in diabetes type I, on GVHD and in aplastic anemia.
2. Embryonic Stem Cell – induction of the hematopoietic and endothelial differentiation with the objective to get therapeutically amounts of platelets, erythrocyte and endothelial cells.
3. Generation of lineages of Induced Pluripotent Stem Cells (iPS) from fibroblasts of hemophilic and osteogenesis imperfect patients for the study of mechanisms and establishment of pluripotent cellular lineages with corrected genome aiming future therapeutic use.
4. Direct reprogramming of fibroblasts or keratinocytes from normal individuals into hematopoietic stem cell aiming at studies of mechanisms and of ideal combination of genes for induction of the reprogramming.
5. Molecular and genetic mechanisms involved in the epithelial-mesenchymal transition (EMT), endothelial-mesenchymal (EndMT) and their reverses MET and MEndT, mainly in the process of induction of the tumor metastasis and in the generation of cancer stem cells.
6. Production of recombinant proteins, mainly of the coagulation factors VIII, IX and VII.
7. Development of molecular tests for detection of viral illnesses transmitted by cellular products.

Lab Staff Molecular Biology and Gene Expression
Simone Kashima Haddad, PhD – Laboratory Coordinator
Evandra Strazza Sandoval, MSc PhD Student, Technician
Virginia Mara de Deus Wagatsuma, PhD, Technician
Fernanda Ursoli MSc Student, Technician
Maria Augusta Sartori Post-Doc/CNPq
Svetoslav Nanev Slavov Post-Doc/FAPESP
Tathiane Maistro Malta Pereira, MSc PhD Student/FAPESP
Mauricio Cristiano Rocha Junior PhD Student/FAPESP
Mariana Tomazini Pinto PhD Student/CNPq
Katia Kaori Otaguiri MSc Student/FAPESP
Mayra Dorigan MSc Student
Carmen Cecilia Oliva Simão SecretaryCell Culture and Therapy
Maristela Delgado Orellana, MsC – Laboratory Coordinator
Gil Cunha De Santis, PhD Medical Doctor
Hudson Bezerra MSc Student
Sâmia Rigotto Caruso MSc Student
Karina Rosa Solano Technician
Taísa Risque Fernandes TechnicianGene Transfer
Simone Kashima Haddad, PhD – Laboratory Coordinator
Ricardo Bonfim-Silva, MSc PhD Student/FAPESP
Lucas E. Botelho de Souza MSc Student/CNPq
Everton de B. Oliveira Costa MSc Student /CNPq
Daianne M. A. de Carvalho CTC/FAPESP fellow
Angelo Luis Caron IC Student
Hudson Bezerra MSc Student

Cytometry
Patricia Viana Bonini Palma – Laboratory Coordinator
Daiane Fernanda dos Santos, MSc Technician
Camila C. de O. Menezes Bonaldo Technician

Confocal Microscopy
Danielle A. Rosa de Magalhães, PhD Technician
Josiane Serrano Borges DTI3, CNPq fellow

Recombinant Protein Expression
Virginia Picanço Castro, PhD
Luiza Cunha Junqueira Reis MSc Student/FAPESP
Aline de Souza Bonfim MSc Student/CNPq
Marcela C. Corrêa de Freitas, MSc PhD Student/FAPESP
Maria Florencia Tellechea IC/CNPq
Lilian Figueiredo Moreira PhD Student/Capes
Fillipe Luiz Rosa do Carmo MSc Student/CNPq
Ana Paula Costa de Pontes Professional – CNPq
Mario Soares de Abreu, MSc Technician
Amanda Mizukami Technician (DTI-II)
Robson Luiz Ferraz do Amaral IC/FAPESP
Gustavo Manella Lúcio IC/CNPq
Rafael Paggé Biagio MSc Student

Collaborators Elisa Maria Russo Carbolante, PhD
Universidade de São Paulo
Faculdade de Ciências Farmacêuticas de Ribeirão PretoKamilla Swiech, PhD
Universidade de São Paulo
Faculdade de Ciências Farmacêuticas de Ribeirão PretoMaria Angélica Miglino, PhD
Universidade de São Paulo
Faculdade de Medicina Veterinária e Zootecnia

Claudio Alberto Torres Suazo, PhD
Centro de Ciências Exatas e de Tecnologia
Universidade Federal de São Carlos

Fabíola Attie de Castro, PhD
Universidade de São Paulo
Faculdade de Ciências Farmacêuticas de Ribeirão Preto
Departamento de Análises Clínicas Toxicológicas e Bromatológicas

Ricardo Pasquini. MD, PhD
Universidade Federal do Paraná
Setor de Ciências da Saúde – Departamento de Clínica Médica

International Collaborators Robert A. Weinberg, Ph.D.,
Member Whitehead Institute for Biomedical Research
Professor of Biology, MIT
Director, MIT Ludwig Center for Molecular OncologyGraça D. Almeida-Porada, MD,PhD
Professor of Regenerative Medicine
Wake Forest Institute for Regenerative MedicineGregory J. Riggins, M.D. Ph.D.
Professor of Neurosurgery & Oncology
Irving J. Sherman Research Professor in Neurosurgery
Director, Division of Neurosurgery Research
Facilities Coordination Cytometry
Confocal Microscopy
GMP Culture Laboratory
Large Scale Culture Laboratory
Selected Publications 1. Ferreira, AF; Moura, LG. ; Tojal, I. ; Ambrósio, L; Pinto-Simões, B; Hamerschlak, N; Calin, GA; Ivan, C; Covas, DT; Kashima, S; Castro, FA. ApoptomiRs expression modulated by BCR-ABL is linked to CML progression and imatinib resistance. Blood Cells, Molecules & Diseases (Print), v. 53, p. 47-55, 2014.

2. De Santis, GC; Prata, KL; Rodrigues, RB; Sankarankutty, AK; De Castro e Silva, O; Covas, DT. Blood group O patients require more blood transfusion in orthotopic liver transplantation. Transfusion and Apheresis Science, v. 14, p. 9, 2014.

3. Picanço-Castro, V; Moreira, LF; Kashima, S; Covas, DT. Can Pluripotent Stem Cells Be Used in Cell-Based Therapy?. Cellular Reprogramming, v. 1, p. 140307082255007, 2014.

4. Slavov, SN; Kashima, S; Rocha-Junior, MC; Oliveira, LC; Silva-Pinto, AC; Yamamoto, AY; Covas, DT. Frequent human parvovirus B19 DNA occurrence and high seroprevalence in haemophilic patients from a non-metropolitan blood centre, Brazil. Transfusion Medicine (Print), v. 24, p. 130-132, 2014.

5. Rodrigues, ES; De Macedo, MD; Pinto, MT; Orellana, MD; Rocha Junior, MC; De Magalhães, DAR; Tanaka, Y; Takayanagui, OM; Covas, DT; Kashima, S. HTLV-1 infects human mesenchymal stromal cell in vitro and modifies their phenotypic characteristics. Virology (New York, N.Y. Print), v. 449, p. 190-199, 2014.

6. Perlingeiro Beltrame, M; Malvezzi, M; Bonfim, C; Covas, DT; Orfao, A; Pasquini, R. Immune reconstitution in patients with Fanconi anemia after allogeneic bone marrow transplantation. Cytotherapy (Oxford), v. 1, p. 1, 2014.

7. Malta, TM; Silva, IT; Pinheiro, DG; Santos, ARD; Pinto, MT; Panepucci, RA; Takayanagui, OM; Tanaka, Y; Covas, DT; Kashima, S. Altered Expression of Degranulation-Related Genes in CD8 + T Cells in Human T Lymphotropic Virus Type I Infection. AIDS Research and Human Retroviruses, v. 29, p. 826-836, 2013.

8. Santis, GCD; Ubiali, EMA; Covas, DT. Compassionate use of cell products. Revista Brasileira de Hematologia e Hemoterapia (Impresso), v. 35, p. 144, 2013.

9. Oliveira, FM; Araujo, ARL; Favarin, MDC; Palma, PVB; Rego, EM; Falcão, RP; Covas, DT; Fontes, AM. Differential expression of AURKA and AURKB genes in bone marrow stromal mesenchymal cells of myelodisplastic syndrome: Correlation with G-banding analysis and FISH. Experimental Hematology, v. 41, p. 198-208, 2013.

10. Mizukami, A; Orellana, MD; Caruso, SR; De Lima Prata, K; Covas, DT; Swiech, K. Efficient expansion of mesenchymal stromal cells in a disposable fixed bed culture system. Biotechnology Progress (Print), v. 29, p. 568-572, 2013.

11. Palma, C; Tannous, M; Malta, T; Carbolante, ES; Covas, DT; Picanço-Castro, V. Forced expression of OCT4 influences the expression of pluripotent genes in human mesenchymal stem cells and fibroblasts.. Genetics and Molecular Research, v. 2, p. 1054-1060, 2013.

12. Pinto, MT; Malta, TM; Rodrigues, ES; Pinheiro, DG; Panepucci, RA; Panepucci, RA; Farias, KCRMD; De Paula Alves Sousa, A; Takayanagui, OM; Tanaka, Y; Covas, DT; Kashima, S. Genes related to antiviral activity, cell migration, and lysis are differentially expressed in CD4+ T cells in HAM/TSP patients.. AIDS Research and Human Retroviruses, v. 29, p. 130917035218000, 2013.

13. Brunetta, DM; De Santis, GC; Vilar, FC; Vilar, FC; Brandão, RA; Muniz, RZDA; De Lima, GMN; Amorelli-Chacel, ME; Covas, DT; Machado, AA. Hematological particularities and co-infections in injected drug users with AIDS. The Brazilian Journal of Infectious Diseases (Impresso), v. 13, p. S1413-8670(13)0, 2013.

14. Slavov, SN; Kashima, S; Silva-Pinto, AC; Amarilla, AA; Aquino, VH; Covas, DT. Molecular and clinical evaluation of the acute human parvovirus B19 infection: comparison of two cases in children with sickle cell disease and discussion of the literature. The Brazilian Journal of Infectious Diseases (Impresso), v. 17, p. 97-101, 2013.

15. De Santis, GC; Brunetta, DM; Nardo, M; Oliveira, LC; Souza, FF; Cagnolati, D; Mente, ÊD; Sankarankutty, AK; Covas, DT; De Castro E Silva, O. Preoperative variables associated with transfusion requirements in orthotopic liver transplantation. Transfusion and Apheresis Science, v. 1, p. 1, 2013.

16. Del Lama, LS; De Góes, EG; Petchevist, PCD; Moretto, EL; Borges, JC; Covas, DT; De Almeida, A. Prevention of Transfusion-Associated Graft-versus-Host Disease by Irradiation: Technical Aspect of a New Ferrous Sulphate Dosimetric System. Plos One, v. 8, p. e65334, 2013.

17. Rodrigues, ES; Kashima, S; Picanço-Castro, V; Espanhol, MR; De Andrade, LA; Palma, PV; Kashima, S; Fontes, AM; Covas, DT. Quantitative correlation between transcriptional levels of ER chaperone, peroximal protein and FVIII productivity in human Hek-293 cell line. SpringerPlus, v. 2, p. 328, 2013.