Jose Cesar Rosa
Assistant Professor, Department of Molecular and Cell Biology, Medical School of Ribeirao Preto, University of São Paulo
jcrosa@fmrp.usp.br

Education  

1975-1978 BSc , Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil
1993-1995 MSc, Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil
1996-1998 Ph.D, Analytical Chemistry, Institute of Chemistry of São Carlos, University of São Paulo, Brazil
1999-2001……..Post-doctor fellow, Department of Chemistry, University of New Hampshire, Durham,NH, USA

Research Interests  

Cancer and Stem Cell Biology
Proteomics
Posttranslational modification and cell signaling
Glycobiology

Research Overview  

The major focus of our group is to understand the molecular mechanisms involved in stem cell differentiation and cancer development. We are using cell biology and proteomic analysis to identify and characterize proteins and the role of their posttranslational modifications (phosphorylation and glycosylation) which are involved in biologic process such as cell proliferation, cell differentiation, cell death and cell intercommunication based on protein-protein interaction and carbohydrate-mediated interactions in mesenchymal stem cell and brain cancer.

Lab Staff  

Marcela Gimenez, PhD Student
Rodrigo Cesar Vida, PhD Student
Érika da Silva Czerniz, MSc Student
Thaiene Aizza, Scientific Initiation
Nicolas Augusto Grine do Carmo, Pre-Scientific Initiation
Mayara Hellen Lemes Rossato, Pre-Scientific Initiation

Technicians

Clarice Izumi, PhD
Maria Helena Dias Martins, BSc
Vitor Antonio Darin, MSc.


Collaborators  

Suely S. K. Nagashi Marie, PhD
Faculdade de Medicina de São Paulo
Universidade de São Paulo
Laboratório de Biologia Molecular, Neurology

Roger Chammas, PhD
Universidade de São Paulo
Laboratório de Experimental Oncology

Adalberto Luiz Rosa, PhD
Faculdade de Odontologia de Ribeirão Preto, USP

Maria Aparecida Nagai, PhD
Faculdade de Medicina de São Paulo

International Collaborators  

 

Facilities Coordination   Protein Chemistry Center and Mass Spectrometry Facility.
Selected Publications  

1.Fonseca FP, Soares-Costa A, Ribeiro AF, Rosa JC, Terra WR, Henrique-Silva F. Recombinant expression, localization and in vitro inhibition of midgut cysteine peptidase (Sl-CathL) from sugarcane weevil, Sphenophorus levis. Insect Biochem Mol Biol. 2012 Jan;42(1):58-69.

2.Cologna CT, Peigneur S, Rosa JC, Selistre-de-Araujo HS, Varanda WA, Tytgat J, Arantes EC. Purification and characterization of Ts15, the first member of a new α-KTX subfamily from the venom of the Brazilian scorpion Tityus serrulatus. Toxicon. 2011 Jul;58(1):54-61.

3.Silva MT, Ambrósio DL, Trevelin CC, Watanabe TF, Laure HJ, Greene LJ, Rosa JC, Valentini SR, Cicarelli RM. New insights into trypanosomatid U5 small nuclear ribonucleoproteins. Mem Inst Oswaldo Cruz. 2011 Mar;106(2):130-8.

4. Mendonça-Franqueiro, E. d. P., Alves-Paiva, R. d. M., Sartim, M. A., Callejon, D. R., Paiva, H. H., Antonucci, G. A., Rosa, J. C., Cintra, A. C. O., Franco, J. J., Arantes, E. C., DIAS-BARUFFI, M., Sampaio, S. V. Isolation, functional, and partial biochemical characterization of galatrox, an acidic lectin from Bothrops atrox snake venom. Acta Biochimica et Biophysica Sinica. , p.1 – 12, 2011.

5. Lico, D.T.P., ROSA, J.C., ROSA, J. C., DeGiorgis, J.A., deVasconcelos, E.J.R., Casaletti, L., TAUHATA, S. B. F., BAQUI, M. M. A., Fukuda, M., Moreira, J.E., LARSON, R.E. A NOVEL 65 kDa RNA-BINDING PROTEIN IN SQUID PRESYNAPTIC TERMINALS.. Neuroscience. , v.166, p.73 – 83, 2010.

6. BARROS, B. H. R., SILVA, S. H., MARQUES, E. R., ROSA, J.C., YATSUDA-NATSUI, A. P., ROBERTS, D. W., BRAGA, G. U. L. A proteomic approach to identifying proteins differentially expressed in conidia and mycelium of the entomopathogenic fungus Metarhizium acridum. Fungal Biology. , v.114, p.572 – 579, 2010.

7. BOTELHO-MACHADO, C., CABRAL, F.J., SOARES, C. S., MOREIRA, E. B. C., MORAIS, E. R., MAGALHÃES, L. G., GOMES, M. S., GUERRA-SÁ, R., ROSA, J.C., RULLER, R., WARD, R. J., RODRIGUES, V. Characterization and mRNA expression analysis of PI31, an endogenous proteasome inhibitor from Schistosoma mansoni.. Parasitology Research. , v.x, p.x – x, 2010.

8. Nardi, Daniela T., ROSA, J.C., Jubilut, Guita N., MIRANDA, A., Nascimento, Nanci, Nakaie, Clovis R. Gamma Ray Irradiation of the Vasoactive Peptide Bradykinin Reveals a Residue- and Position-Dependent Structural Modification. International Journal of Peptide Research and Therapeutics (Print). , v.16, p.71 – 78, 2010.

9. SPILLER, F., COSTA, C., SOUTO, F. O., VINCHI, F., Mestriner, F. L. A. C., LAURE, H. J., ALVES-FILHO, J. C., FREITAS, A., Rosa, J. C., Ferreira, S. H., ALTRUDA, F., HIRSCH, E., GREENE, L. J., TOLOSANO, E., CUNHA, F. Q. Inhibition of Neutrophil Migration by Hemopexin Leads to Increased Mortality Due to Sepsis in Mice. American Journal of Respiratory and Critical Care Medicine. , p.1 – 55, 2010.

10. GIMENEZ, M., SOUZA, V. C. O., IZUMI, C., BARBIERi, M. R., CHAMMAS, R., OBA-SHINJO, S. M., Uno, M, MARIE, S. K. N., ROSA, J.C. Proteomic analysis of low to high grade astrocytomas reveals an alteration of the expression level of RKIP and nucleophosmin. Proteomics (Weinheim. Print). , v.10, p.2812 – 2821, 2010.
11. Viana, Pollyanna A., REZENDE, Sebastiao Tavares de, Passos, F. M. L., Oliveira, Jamil S., Teixeira, K. N., Santos, Alexandre M. C., Bemquerer, Marcelo P., ROSA, J.C., Santoro, Marcelo M., GUIMARAES, V. M. Debaryomyces hansenii UFV-1 Intracellular alpha-Galactosidase Characterization and Comparative Studies with the Extracellylar Enzyme. Journal of Agricultural and Food Chemistry. , v.57, p.2515 – 2522, 2009.

12. Lopes, J.L.S., Valadares, N.F., Moraes, D.I., ROSA, J.C., ARAÚJO, Heloisa Sobreiro Selistre de, BELTRAMINI, L. M. Physico-chemical and antifungalproperties of protease inhibitors from Acacia plumosa. Phytochemistry. , v.70, p.871 – 879, 2009.

13. THOMAZELLA, G. G., Da Silva, I., LAURE, H. J., ROSA, J.C., CHAMMAS, R., Wiker, H. G., SOUZA, G. A., GREENE, L. J. Proteomic analysis of total cellular proteins of human neutrophils. Proteome Science. , v.7, p.32 – 40, 2009.

14. SOUZA, F. H. M., NASCIMENTO, C. V., ROSA, J.C., MASUI, D. C., LEONE, F. A., JORGE, J. A., FURRIEL, R. P. M. Purification and biochemical characterization of a mycelial glucose and xylose-stimulated beta-glucosidase from the thermophilic fungus Humicola insolens. Process Biochemistry. , v.45, p.272 – 278, 2009.

15. Silva, T.M., MICHELIN, M., Damasio, A.R.L., Maller, A., Almeida, F.B.R., RULLER, R., WARD, R. J., ROSA, J.C., JORGE, J. A., Terenzi, H. F., POLIZELI, M. L. T. M. Purification and biochemical characterization of a novel alpha-glucosidase from Aspergillus niveus. Antonie van Leeuwenhoek (Gedrukt). , v.96, p.569 – 578, 2009.

16. ALVES, R.M., Antonucci, G. A., Paiva, H. H., Cintra, A. C. O., Franco, J. J., Mendonça-Franqueiro, E. d. P., DORTA, D.J., GIGLIO, J. R., ROSA, J.C., FULY, A. L., DIAS-BARUFFI, M., SOARES, A.M., Sampaio, S. V. Evidence of caspase-mediated apoptosis induced by l-amino acid oxidase isolated from Bothrops atrox snake venom.. Comparative Biochemistry and Physiology. A, Molecular & Integrative Physiology. , v.151, p.542 – 550, 2008.

17. Mestriner, F. L. A. C., SPILLER, F., LAURE, H. J., SOUTO, F. O., B.M. Tavares-Murta, ROSA, J.C., A. Basile-Filho, FERREIRA, S.H., GREENE, L. J., CUNHA, F. Q. Acute-phase protein alpha-1-acid glycoprotein mediates neutrophil migration. PNAS. Proceedings of the National Academy of Sciences of the United States of America. , v.104, p.19595 – 19600, 2007.

18. Porto, C.A.C., Oliveira, L.L., Ferraz, L.C., Ferraz, L.E., Thomaz, S.M., ROSA, J.C., BARREIRA, M. C. R. A. Isolation of bovine immunoglobulins resitant to peptic digestion: new perspectives in the prevention of failure in passive immunization of neonatal calves. Journal of Dairy Science. , v.90, p.955 – 962, 2007.