Center for Cell-based Therapy – Page 80 – Centro de Terapia Celular

Lewis Joel Greene

		Lewis Joel Greene Full Professor of Cell and Molecular Biology (retired) FMRP/USP Supervisor of the Center for Protein Chemistry, at Regional Blood Center of Ribeirão Preto Volunteer head teacher (senior associate) of FMRP/USP ljgreene@fmrp.usp.br PubMed Scopus ResearchID Scielo Scholar
Education		1951-1955 B.A (magna cum laude), Amherst College, Amherst Massachusetts 1955-1962 Ph D, Rockefeller University, C.H.W. HIRS and G.E. Palade (Research advisors) 1990 Full Professor, University of São Paulo, Brazil
Research Interests		Structure and function of proteins Protein- protein interactions Proteomics as an entry to cell biology Molecular basis of cancer Molecular basis of sepsis
Research Overview		The recent focus of our group has been to understand the molecular mechanisms involved in the development of diseases and cancer . We are collaborating with biologists, clinicians and chemists using the methodologies of protein chemistry and molecular biology to identify biomarkers for diagnosis and prognosis and to understand the processes involved.
Lab Staff		José César Rosa, PhD – Laboratory Collaborator
Collaborators		Carolina Hassibe Thomé, Pos Doc Germano Aguiar Ferreira, Doctoral Student José César Rosa Faculdade de Medicina de Ribeirão Preto – Universidade de São Paulo Centro de Química de Proteínas, Hemocentro de Ribeirão Preto, SP jcrosa@fmrp.usp.br Andréia Machado Leopoldino Departamento de Análises Clínicas, Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo andreiaml@usp.br Dimas Tadeu Covas Faculdade de Medicina de Ribeirão Preto – Universidade de São Paulo Centro Regional de Hemoterapia de Ribeirão Preto dimas@fmrp.usp.br Vitor Marcel Faça Prof. Dr. da Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo Department of Biochemistry and Immunology vitor.faca@fmrp.usp.br Eduardo Magalhães Rego Departamento de Clínica Médica da Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo edumrego@hotmail.com Wilson Araújo da Silva Júnior Departamento de Genética da Faculdade de Medicina de Ribeirão Preto – Universidade de São Paulo wilsonjr@usp.br Roger Chammas Professor Titular, Faculdade de Medicina, Universidade de São Paulo – USP Departamento de Radiologia rchammas@lim24.fm.usp.br
International Collaborators
Facilities Coordination		Mass Spectrometry and Protein Chemistry Lab
Selected Publications		1. SOBRAL, L. M. ; SOUSA, L.O. ; COLETTA, R. D. ; CABRAL, H. ; GREENE, L. J. ; TAJARA, E.H. ; GUTKIND, J. S. ; CURTI, C. ; Leopoldino, A. M. . Stable SET knockdown in head and neck squamous cell carcinoma promotes cell invasion and the mesenchymal-like phenotype in vitro, as well as necrosis, cisplatin sensitivity and lymph node metastasis in xenograft tumor models. Molecular Cancer , v. 13, p. 32, 2014. 2. Thomé, C. H. ; DOS SANTOS, G. A. ; FERREIRA, G. A. ; SCHEUCHER, P. S. ; IZUMI, C. ; Leopoldino, A. M. ; SIMAO, A. M. ; CIANCAGLINI, P. ; DE OLIVEIRA, K. T. ; CHIN, A. ; HANASH, S. M. ; FALCAO, R. P. ; REGO, E. M. ; Greene, L. J. ; Faça, V. M. . Linker for Activation of T-cell Family Member2 (LAT2) a Lipid Raft Adaptor Protein for AKT Signaling, Is an Early Mediator of Alkylphospholipid Anti-leukemic Activity. Molecular & Cellular Proteomics , v. 11, p. 1898-1912, 2012. Citações: 3\| 4 3. Fontes, A. M. ; Melo, F.U.F. ; Greene, L.J. ; Faça, V. M. ; Lin, Y. ; Gerson, S.L. ; Covas, D. T. . Production of human factor VIII-FL in 293T cells using the bicistronic MGMT(P140K)-retroviral vector. Genetics and Molecular Research , v. 11, p. 775-789, 2012. 4. Miranda, H. C. ; HERAI, R. H. ; Thomé, C. H. ; Gomes, G. G. ; PANEPUCCI, R. A. ; ORELLANA, M. D. ; Covas, D. T. ; Muotri, A. R. ; Greene, L.J. ; Faça, V. M. . A quantitative proteomic and transcriptomic comparison of human mesenchymal stem cells from bone marrow and umbilical cord vein. Proteomics (Weinheim. Print) , v. 12, p. 2607-2617, 2012. Citações: 3\| 6 5. Spiller, F. ; FERREIRA, S. H. ; Greene, L. J. ; Rosa, J. C. ; Altruda, F. ; Souto, F. O. ; Hirsch, E. ; Mestriner, F. L. A. C. ; Cunha, F. Q. ; Tolosano, E. ; Alves-Filho, J. C. ; Laure, H. J. ; Freitas, A. ; Costa, C. ; Vinchi, F. . Inhibition of Neutrophil Migration by Hemopexin Leads to Increased Mortality Due to Sepsis in Mice. American Journal of Respiratory and Critical Care Medicine , v. 183, p. 922-931, 2011. Citações: 7\| 7 6. Santos, G. A. S. ; Thomé, C. H. ; FERREIRA, G.A. ; YONEDA, J. S. ; NOBRE, T. M. ; Daghastnali, K.R.P. ; SCHEUCHER, P. S. ; TEIXEIRA, H. L. ; Constantino, M. G. ; DE OLIVEIRA, K. T. ; Faça, V. M. ; FALCAO, R. P. ; GREENE, L. J. ; REGO, E. M. ; CIANCAGLINI, P. . Interaction of 10-(octyloxy) decyl-2-(trimethylammonium) ethyl phosphate with mimetic membranes and cytotoxic effect on leukemic cells. Biochimica et Biophysica Acta. Biomembranes , v. 1798, p. 1714-1723, 2010. Citações: 4\| 5 7. Mestriner, F. L. A. C. ; Spiller, F. ; Laure, H. J. ; Souto, F. O. ; TAVARES-MURTA, B.M. ; Rosa, J. C. ; Basile Filho, A. ; FERREIRA, S. H. ; GREENE, L. J. ; Cunha, F. Q. . Acute-phase protein α -1-acid glycoprotein mediates neutrophil migration failure in sepsis by a nitric oxide-dependent mechanism.. Proceedings of the National Academy of Sciences of the United States of America (Online) , v. 104, p. 19595-19600, 2007. Citações: 20\| 22 8. SOUZA, G. A. ; GODOY, L.M.F. de ; TEIXEIRA, V. R. ; OTAKE, A. H. ; SABINO, A. ; Rosa, J. C. ; Dinarte, A. R. ; Pinheiro, D. G. ; Silva Jr, W. A. ; EBERLIN, M. N. ; Chammas, R. ;GREENE, L. J. . Proteomic and SAGE profiling of murine melanoma progression indicates the reduction of proteins responsible for ROS degradation. Proteomics, Inglaterra, v. 6, n.5, p. 1460-1470, 2006. Citações: 22\| 22

Flávio Vieira Meirelles

		Flávio V. Meirelles Full Professor of Cell Biology & Development, FZEA/USP Member of the Coordinating Committee (Veterinary Medicine) of FAPESP Associate editor of Genetics And Molecular Research meirellf@usp.br PubMed Scopus ORCID ResearcherID Scholar
Education		1989-1993 Graduated in Veterinary Medicine, São Paulo State University, Brazil 1994-1996 Master Thesis in Reproduction, University of Montreal, Canada 1997-1999 PhD in Genetics, Medical Scholl of Ribeirão Preto, University of São Paulo 2004 Full Professor, University of São Paulo, USP, Brazil
Research Interests		Cytoplasmic inheritance Nuclear Reprograming Animal Genetics Mitochondrial genome Embryonic genome activation
Research Overview		Since the last 20 years we have focused on understanding the process of cytoplasmic inheritance. We have successfully created animal models to study mtDNA inheritance, and its related cell death process during early embryo development, which allowed us to develop a patent on SCNT animal production. We also dedicated to understand the process of cytoplasmic inheritance and its effect on nuclear reprograming, especially on the imprinting modifications after in vitro manipulation such as cloning or reprograming somatic cells. Recently we developed an interesting method for depletion of specific organelles or compounds in oocytes, zygotes or cells that allowed us to test for their effect on embryogenesis and to introduce other organelles or compounds (we did it on mitochondria and we are now testing on lipid granules) in order to partially control their inheritance. Within the next 11 years, we will together with the CTC group continue to work on this area willing to evaluate the production of reprogrammed cells to autologous cell therapy and to control the cytoplasmic inheritance in order to apply to cell based therapy.
Lab Staff		Pos Docs Marcos Roberto Chiaratti, PhD Lilian de Jesus Oliveira,PhD José Rodrigo V. Pimentel, PhD PhD Fabiana Fernandes Bressan,MSc Reno Roldi Araujo, MSc Rodrigo Barreto, MSc Rafael Sampaio, MSc Juliano Sangalli, MSc Pedro Ratto Pires, MSc MSc Lais Vicari Pessoa, DVM Gabriella Mamede Andrade, DVM
Collaborators		Claudia Lima Verde Leal, PhD Universidade Federal de São Paulo LMMD Carlos Eduardo Ambrósio, PhD Universidade Federal de São Paulo LMMD Felipe Perecin, PhD Universidade Federal de São Paulo LMMD Heidge Fukumasu Universidade Federal de São Paulo LMMD Maria Angélica Miglino Universidade Federal de São Paulo FMVZ – Programa de Pós Graduação em Anatomia
International Collaborators		Joanna Poulton, PhD Professor of Genetics Univ. of Oxford, Lawrence Charles Smith, PhD Full Professor – Canadian Chair of Animal Cloning Université de Montréal Willian Allan King Full Professor – Canadian Chair of Animal Biotechnology Guelph – ON Canada
Facilities Coordination		Embryo Manipulation Facilities including IVF Laboratory, Cell sorting, Basic Molecular Biology lab. Animal Hospital Facility dedicated for Innovative Therapy.
Selected Publications		1. BRESSAN, Fabiana Fernandes ; Dos Santos Miranda, Moyses ; PERECIN, Felipe ; De Bem, Tiago Henrique ; Pereira, Flavia Thomaz Verechia ; Russo-Carbolante, Elisa Maria ; Alves, Daiani ; Strauss, Bryan ; Bajgelman, Marcio ; Krieger, José Eduardo ; Binelli, Mario ; Meirelles, Flavio Vieira . Improved Production of Genetically Modified Fetuses with Homogeneous Transgene Expression After Transgene Integration Site Analysis and Recloning in Cattle. Cellular Reprogramming (Formerly Cloning and Stem Cells), v.13 (1) p.29-36, 2011. 2. CHIARATTI, Marcos Roberto; FERREIRA, Christina Ramires; PERECIN, Felipe … Meirelles, Flávio Vieira . Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. Reprod. BioMedicine Online, v. 22, p. 172-183, 2011. 3. Suzuki Jr, João ; Therrien, Jacinthe ; Filion, France ; Lefebvre, Rejean …Perecin, Felipe ; Meirelles, Flávio V. ; Smith, Lawrence Charles . Loss of Methylation at H19 DMD Is Associated with Biallelic Expression and Reduced Development in Cattle Derived by Somatic Cell Nuclear Transfer. Biology of Reproduction, v. 84, p. 947-956, 2011. 4. Bressan, Fabiana Fernandes; Perecin, Felipe; Sangalli, Juliano Rodrigues ; Meirelles, F. V. Reprogramming somatic cells: pluripotency through gene induction and nuclear transfer. Acta Scientiae Veterinariae, v. 39, p. s83-s95, 2011. 5. De Bem, T. H. C. ; Chiaratti, M. R. ; Rochetti, R. ; Bressan, F. F. ; Sangalli, J. R. ; Miranda, M. S. ; Pires, P. R. L. ; Schwarz, K.R.L. ; Sampaio, R. V. ; Fantinato Neto, P. ; Pimentel, J. R. V. ; Perecin, F. ; Smith, L. C. ; Meirelles, F. V. ; Leal, C. L. V. . Viable calves produced by somatic nuclear transfer using meiotic-blocked oocytes. Cellular reprogramming, v. 13, p. DOI: 10.1089/c, 2011. 6. Chiaratti, M R ; Meirelles, F. V. ; Wells, D ; Poulton, J . Therapeutic treatments of mtDNA diseases at the earliest stages of human development. Mitochondrion (Amsterdam. Print), v. 11, p. 820-828, 2011. 7. Ferreira, C. R. ; Burgstaller, J. P. ; Perecin, F. ; Garcia, J. M. ; Chiaratti, M. R. ; Meo, S. C. ; Muller, M. ; Smith, L. C. ; Meirelles, F. V. Pronounced Segregation of Donor Mitochondria Introduced by Bovine Ooplasmic Transfer to the Female Germ-Line. Biology of Reproduction, v. 82, p. 563-571, 2010. 8. Meirelles, Flávio V. ; Birgel, Eduardo H. ; PERECIN, Felipe Et Al., Delivery of cloned offspring: experience in Zebu cattle. Reprod., Fertility and Dev. v. 22, p. 88, 2010. 9. Chiaratti, M. R. ; Meirelles, F. V. . Mitochondrial DNA Copy Number, a Marker of Viability for Oocytes. Biology of Reproduction, v. 83, p. 1-2, 2010. 10. Ferreira, Christina Ramires ; Saraiva, Sérgio Adriano ; Catharino, Rodrigo Ramos ; Garcia, Jerusa Simone ; Gozzo, Fábio César ; Sanvido, Gustavo Braga ; Santos, Luiz Fernando Arruda ; Turco, Edson Guimarães Lo ; Pontes, José Henrique Fortes ; Basso, Andréa Cristina ; Bertolla, Ricardo Pimenta ; Sartori, Roberto ; Guardieirio, Monique Mendes ; Perecin, Felipe ; Meirelles, F. V; Eberlin, Marcos Nogueira . Single embryo and oocyte lipid fingerprinting by mass spectrometry. Journal of Lipid Research (Print), v. 51, p. 1218-1227, 2010. 11. Chiaratti, Marcos Roberto ; Ferreira, Christina Ramires ; Meirelles, Flávio Vieira ; et al. Xenooplasmic Transfer between Buffalo and Bovine Enables Development of Homoplasmic Offspring. Cellular Reprogramming (Formerly “Cloning and Stem Cells”), v. 12, p. 231-236, 2010. 14. Poulton, Joanna ; Chiaratti, Marcos R. ; Meirelles, Flávio V. ; Kennedy, Stephen ; Wells, Dagan ; Holt, Ian J. ; Suomalainen, Anu . Transmission of Mitochondrial DNA Diseases and Ways to Prevent Them. PLoS Genetics, v. 6, p. e1001066-08, 2010. 15. Perecin, F. ; Meo, S.C. ; Yamazaki, W. ; Ferreira, C.R. ; Merighe, G.K.F. ; Meirelles, F.V. ; Garcia, J.M. . Imprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes. Gen. and Mol. Res., v. 8, p. 76-85, 2009. 16. Dos Santos Miranda, Moysés ; BRESSAN, Fabiana Fernandes ; Zecchin, Karina Gottardello ; Vercesi, Anibal Eugênio ; MESQUITA, Ligia Garcia ; Fonseca Merighe, Giovana Krempel ; KING, William Allan ; Ohashi, Otávio Mitio ; Pimentel, José Rodrigo Valim ; PERECIN, Felipe ; Meirelles, Flávio Vieira . Serum-Starved Apoptotic Fibroblasts Reduce Blastocyst Production but Enable Development to Term after SCNT in Cattle. Cloning and Stem Cells, v. 11, p. 1-9, 2009. 17 Chiaratti, M. R. ; Bressan, F. F. ; Ferreira, C. R. ; Caetano, A. R. ; Smith, L. C. ; Vercesi, A. E. ; Meirelles, F. V. . Embryo Mitochondrial DNA Depletion Is Reversed During Early Embryogenesis in Cattle. Biology of Reproduction, v. 82, p. 76-85, 2009. 18. Ferreira, E.M. ; Vireque, A.A. ; Adona, P.R. ; Meirelles, F.V. et al., Cytoplasmic maturation of bovine oocytes: Structural and biochemical modifications and acquisition of developmental competence. Theriogenology, v. 71, p. 836-848, 2009. 19. BIASE, Fernndo Henrique ; MARTELLI, L ; MERIGHE, Giovana Krempel Fonseca ; BIASE, Weruska Karyna Freitas Santos ; MIRANDA, Moysés Dos Santos ; SMITH, L. C. ; Meirelles, F. V.A retrospective model of oocyte competence: global mRNA and housekeeping transcripts are not associated with in vitro developmental outcome.. Zygote (Cambridge), v. 17, p. 289-295, 2009. 20. Bressan, F.F. ; De Bem, T.H.C. ; Perecin, F. ; Lopes, F.L. ; Ambrosio, C.E. ; Meirelles, F.V. ; Miglino, M.A. . Unearthing the Roles of Imprinted Genes in the Placenta. Placenta (Eastbourne), v. 30, p. 823-834, 2009.

Eduardo Magalhães Rego

		Eduardo Magalhães Rego Professor of Hematology/Oncology, Medical School of Ribeirão Preto, University of São Paulo Head of the Hematology and Clinical Oncology Service of the University Hospital (HC-FMRP) Coordinator in Brazil of the International Consortium on Acute Leukemias which is sponsored by the American Society of Hematology Member of the Board of Directors of the Brazilian Association of Hematology (ABHH) Editor of the Brazilian Journal of Medical and Biological Research and Associate Editor of Blood and Annals of Hematology emrego@hcrp.fmrp.usp.br PubMed Scopus ORCID ResearcherID Scholar
Education		1983-1988 Doctor of Medicine (M.D.) Medical School of Ribeirão Preto of the University of São Paulo 1989-1992 Medical Residence/ Fellowship in Hematology, University Hospital, Medical School of Ribeirão Preto of the University of São Paulo 1993-1997 Doctor of Philosophy (Ph.D.) Medical School of Ribeirão Preto of the University of São Paulo 1998-2001 Post-Doctoral Fellowship Memorial Sloan-Kettering Cancer Center, New York, USA 2002 Full Professor, University of São Paulo, USP, Brazil 2005 PhD in Medicine, Fox Chase Cancer Center, FCCC, USA
Research Interests		Hematological malignancies, in particular acute leukemias Leukemogenesis Animal models of acute leukemia Leukemia and cancer stem cells BV-CDI FAPESP
Research Overview		The main focus of our group is to integrate basic and clinical research on acute leukemias, in special acute myeloid leukemias. In order to have a better insight on the molecular basis of leukemogenesis, we have been studying acute promyelocytic leukemia (APL) as a model. We have been addressing the following questions: 1. Which cell subpopulation acts the leukemia initiating cells and how is quiescence and energy generation regulated in this subset; 2. Which microRNAs act as key regulators of differentiation in APL blasts and whether their expression profile are associated with treatment outcome; 3. Does the expression of transcriptinally active and inactive isoforms of the p73 gene affect the response to ATRA and if so how? 4. What is the role of microparticles and annexin II expression in APL-associated coagulopathy; 5. Can the molecular monitoring of the PML/RARA fusion gene using RQ-PCR technology predict relapse better than the routinely used RT-PCR method?; 6. Is leukemia relapse is associated with additional mutagenic events? 7. In leukemia prone syndromes, such as dyskeratosis congenita, which pathways confer survival advantage to hematopoietic progenitors and are associated in malignant transformation. Finally, our group is coordinating in Brazil the International Consortium on APL, which is a trial aiming to improve the treatment outcome of APL patients in developing countries.
Lab Staff		1. PhD students: a. Ana Paula A. de L. Lange b. Antonio R. L. de Araújo c. Helder Henrique Paiva d. Sarah Cristina Bassi 2. MsC students: a. Katarina Holanda 3. Post-docs: a. Priscila Santos Scheucher b. Guilherme Augusto Silva dos Santos Molecular Biology Technicians Ana Sílvia Gouveia Lima
Collaborators		Andréia M Leopoldino, PhD Depto de Análises Clínicas, Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo
International Collaborators		Dr Davide Rugero, Associate Professor Helen Diller Family Cancer Research Center University of California, San Francisco (UCSF) Mission Bay Campus 1450 3rd Street Room HD 386, MC 3110 San Francisco, CA 94158 Tel: 415/514-9755 Fax: 415/514-4826 davide.ruggero@ucsf.edu Prof. Ellen Solomon, Prof. David Grimwade, King’s College London School of Medicine Department of Medical and Molecular Medicine Floor 8 Tower Wing Guy’s Hospital London SE1 9RT Telephone : 0207 1882579 david.grimwade@genetics.kcl.ac.uk http://www.kcl.ac.uk/medicine/research/divisions/gmm/sections/clusters/cancer/solomon/index.aspx http://www.kcl.ac.uk/medicine/research/divisions/gmm/sections/clusters/cancer/grimwade/pgrimwade.aspx Prof. Hau C.Kwaan, Marjorie C. Barnett Professor in Hematology-Oncology Professor of Medicine Northwestern University Feinberg School of Medicine Room 8258, Olson Pavilion 710 N.Fairbanks Court, Chicago, IL. 60611 h-kwaan@northwestern.edu http://fsmweb.northwestern.edu/faculty/FacultyProfile.cfm?xid=14961 Prof. Stefan Bohlander, Department of Medicine III, Universität München, 81377 Munich, Germany bohlander@helmholtz-muenchen.de http://www.sfb684.med.uni-muenchen.de/projects/a6bohlander/index.html
Facilities Coordination		Flow Cytometry and Sorting Facility, And Small Animals Irradiator Facility, Medical School of Ribeirão Preto, University of São Paulo, FAPESP EMU – grant 09/54218-1
Selected Publications		Benicio MTL et al. Evaluation of the European LeukemiaNet recommendations for predicting outcomes of patients with acute myeloid leukemia treated in low- and middle-income countries (LMIC): A Brazilian experience. Leuk Res. 2017; 60:109-114.. Corrêa de Araujo Koury L et al. Treating acute promyelocytic leukaemia in Latin America: lessons from the International Consortium on Acute Leukaemia experience. Br J Haematol. 2017;177(6):979-983. Bittencourt R et al. Guidelines on the treatment of acute myeloid leukemia: Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular: Project guidelines: Associação Médica Brasileira – 2015. Rev Bras Hematol Hemoter. 2016; 38(1):58-74. Mota JM et al. Post-Sepsis State Induces Tumor-Associated Macrophage Accumulation through CXCR4/CXCL12 and Favors Tumor Progression in Mice. Cancer Immunol Res. 2016;4(4):312-22. Lucena-Araujo AR et al. High ΔNp73/TAp73 ratio is associated with poor prognosis in acute promyelocytic leukemia. Blood. 2015; 12;126(20):2302-6. Sanz MA et al. All-trans retinoic acid with daunorubicin or idarubicin for risk-adapted treatment of acute promyelocytic leukaemia: a matched-pair analysis of the PETHEMA LPA-2005 and IC-APL studies. Ann Hematol. 2015; 94(8):1347-56. Rego EMet al. Improving acute promyelocytic leukemia (APL) outcome in developing countries through networking, results of the International Consortium on APL. Blood. 2013; 121(11):1935-43. dos Santos GA et al. (+)α-Tocopheryl succinate inhibits the mitochondrial respiratory chain complex I and is as effective as arsenic trioxide or ATRA against acute promyelocytic leukemia in vivo. Leukemia 2012;26(3):451-60. Yoon A et al. Impaired control of IRES-mediated translation in X-linked dyskeratosis congenita. Science. 2006;312(5775):902-6.10. Rego EM et al. Retinoic acid (RA) and As2O3 treatment in transgenic models of acute promyelocytic leukemia (APL) unravel the distinct nature of the leukemogenic process induced by the PML-RARalpha and PLZF-RARalpha oncoproteins. Proc Natl Acad Sci U S A. 2000;97(18):10173-8.

Dimas Tadeu Covas

		Dimas Tadeu Covas Coordinator of the Center for Cell-based Therapy Director of the Instituto Butantan Full Professor of Clinical Medicine – Hematology and Transfusion Medicine Director-President of Regional Blood Center of Ribeirão Preto – FMRP-USP Deputy Coordinator of the National Institute of Science and Technology in Stem Cells and Cell Therapy in Cancer President of the Brazilian Association of Hematology and Hemotherapy Full Member of the Brazilian Technical Commission on Bio-security (CTNBio – 2008/2011) Associate Member: American Society of Hematology and European Hematology Association Full Member of the Technical Board of Hematology for Federal Council of Medicine (CFM) Coordinator of the Postgraduate Course – Professional Master’s in Hemotherapy and Biotechnology, Medical School of Ribeirão Preto – USP, Department of Medical Clinic Full Member of the Advisory Committee of the Strategic Committee for Transplantation and Organ Donation Affairs of the Ministry of Health Member of the Technical Chamber of the Blood and Hemodynamic Coordination of the Ministry of Health dimas@fmrp.usp.br PubMed Scopus ORCID ResearchID Scholar
Education		1976-1981 Graduated in Medicine, Medical School of Ribeirão Preto, University of São Paulo, Brazil 1982 Rotating Resident in Clinical Medicine, University Hospital of Faculty of Medicine of Ribeirão Preto, University of São Paulo, Brazil 1983-1984 Resident in Hematology/Hemotherapy, University Hospital of Medical School of Ribeirão Preto, University of São Paulo, Brazil 1984-1986 Master in Medicine, Medical School of Ribeirão Preto, University of São Paulo, Brazil 1986 -1993 PhD in Medicine, Medical School of Ribeirão Preto, University of São Paulo, Brazil 1999 Full Professor, University of São Paulo, Brazil
Research Interests		Somatic and Pluripotent Stem Cells Cell and Gene Therapy Cell Culture Functional Genomics Recombinant Protein Expression Sickle Cell Anemia Blood Transfusion Virology BV-CDI FAPESP
Research Overview		Our main lines of research are: 1. Mesenchymal Stem Cell – isolation, functional characterization, production on a large scale in bioreactors, biodistribution and clinical potential of isolated MSC of diverse tissues as feral adnexa, umbilical cord, vascular fraction of the adipose tissue and bone marrow. We are also developing clinical studies of phase I in diabetes type I, on GVHD and in aplastic anemia. 2. Embryonic Stem Cell – induction of the hematopoietic and endothelial differentiation with the objective to get therapeutically amounts of platelets, erythrocyte and endothelial cells. 3. Generation of lineages of Induced Pluripotent Stem Cells (iPS) from fibroblasts of hemophilic and osteogenesis imperfect patients for the study of mechanisms and establishment of pluripotent cellular lineages with corrected genome aiming future therapeutic use. 4. Direct reprogramming of fibroblasts or keratinocytes from normal individuals into hematopoietic stem cell aiming at studies of mechanisms and of ideal combination of genes for induction of the reprogramming. 5. Molecular and genetic mechanisms involved in the epithelial-mesenchymal transition (EMT), endothelial-mesenchymal (EndMT) and their reverses MET and MEndT, mainly in the process of induction of the tumor metastasis and in the generation of cancer stem cells. 6. Production of recombinant proteins, mainly of the coagulation factors VIII, IX and VII. 7. Development of molecular tests for detection of viral illnesses transmitted by cellular products.
Lab Staff		Molecular Biology and Gene Expression Simone Kashima Haddad, PhD – Laboratory Coordinator Maristela Delgado Orellana, MsC – Laboratory Coordinator Gil Cunha De Santis, PhD Medical Doctor Cytometry Patricia Viana Bonini Palma – Laboratory Coordinator Confocal Microscopy Josiane Serrano Borges DTI3, CNPq fellow Recombinant Protein Expression Virginia Picanço Castro, PhD – Laboratory Coordinator
Collaborators		Elisa Maria Russo Carbolante, PhD Universidade de São Paulo Faculdade de Ciências Farmacêuticas de Ribeirão Preto Kamilla Swiech, PhD Universidade de São Paulo Faculdade de Ciências Farmacêuticas de Ribeirão Preto Maria Angélica Miglino, PhD Universidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia Claudio Alberto Torres Suazo, PhD Centro de Ciências Exatas e de Tecnologia Universidade Federal de São Carlos Fabíola Attie de Castro, PhD Universidade de São Paulo Faculdade de Ciências Farmacêuticas de Ribeirão Preto Departamento de Análises Clínicas Toxicológicas e Bromatológicas Ricardo Pasquini. MD, PhD Universidade Federal do Paraná Setor de Ciências da Saúde – Departamento de Clínica Médica
International Collaborators		Robert A. Weinberg, Ph.D., Member Whitehead Institute for Biomedical Research Professor of Biology, MIT Director, MIT Ludwig Center for Molecular Oncology Graça D. Almeida-Porada, MD,PhD Professor of Regenerative Medicine Wake Forest Institute for Regenerative Medicine Gregory J. Riggins, M.D. Ph.D. Professor of Neurosurgery & Oncology Irving J. Sherman Research Professor in Neurosurgery Director, Division of Neurosurgery Research
Facilities Coordination		Cytometry Confocal Microscopy GMP Culture Laboratory Large Scale Culture Laboratory
Selected Publications		1. Ferreira, AF; Moura, LG. ; Tojal, I. ; Ambrósio, L; Pinto-Simões, B; Hamerschlak, N; Calin, GA; Ivan, C; Covas, DT; Kashima, S; Castro, FA. ApoptomiRs expression modulated by BCR-ABL is linked to CML progression and imatinib resistance. Blood Cells, Molecules & Diseases (Print), v. 53, p. 47-55, 2014. 2. De Santis, GC; Prata, KL; Rodrigues, RB; Sankarankutty, AK; De Castro e Silva, O; Covas, DT. Blood group O patients require more blood transfusion in orthotopic liver transplantation. Transfusion and Apheresis Science, v. 14, p. 9, 2014. 3. Picanço-Castro, V; Moreira, LF; Kashima, S; Covas, DT. Can Pluripotent Stem Cells Be Used in Cell-Based Therapy?. Cellular Reprogramming, v. 1, p. 140307082255007, 2014. 4. Slavov, SN; Kashima, S; Rocha-Junior, MC; Oliveira, LC; Silva-Pinto, AC; Yamamoto, AY; Covas, DT. Frequent human parvovirus B19 DNA occurrence and high seroprevalence in haemophilic patients from a non-metropolitan blood centre, Brazil. Transfusion Medicine (Print), v. 24, p. 130-132, 2014. 5. Rodrigues, ES; De Macedo, MD; Pinto, MT; Orellana, MD; Rocha Junior, MC; De Magalhães, DAR; Tanaka, Y; Takayanagui, OM; Covas, DT; Kashima, S. HTLV-1 infects human mesenchymal stromal cell in vitro and modifies their phenotypic characteristics. Virology (New York, N.Y. Print), v. 449, p. 190-199, 2014. 6. Perlingeiro Beltrame, M; Malvezzi, M; Bonfim, C; Covas, DT; Orfao, A; Pasquini, R. Immune reconstitution in patients with Fanconi anemia after allogeneic bone marrow transplantation. Cytotherapy (Oxford), v. 1, p. 1, 2014. 7. Malta, TM; Silva, IT; Pinheiro, DG; Santos, ARD; Pinto, MT; Panepucci, RA; Takayanagui, OM; Tanaka, Y; Covas, DT; Kashima, S. Altered Expression of Degranulation-Related Genes in CD8 + T Cells in Human T Lymphotropic Virus Type I Infection. AIDS Research and Human Retroviruses, v. 29, p. 826-836, 2013. 8. Santis, GCD; Ubiali, EMA; Covas, DT. Compassionate use of cell products. Revista Brasileira de Hematologia e Hemoterapia (Impresso), v. 35, p. 144, 2013. 9. Oliveira, FM; Araujo, ARL; Favarin, MDC; Palma, PVB; Rego, EM; Falcão, RP; Covas, DT; Fontes, AM. Differential expression of AURKA and AURKB genes in bone marrow stromal mesenchymal cells of myelodisplastic syndrome: Correlation with G-banding analysis and FISH. Experimental Hematology, v. 41, p. 198-208, 2013. 10. Mizukami, A; Orellana, MD; Caruso, SR; De Lima Prata, K; Covas, DT; Swiech, K. Efficient expansion of mesenchymal stromal cells in a disposable fixed bed culture system. Biotechnology Progress (Print), v. 29, p. 568-572, 2013. 11. Palma, C; Tannous, M; Malta, T; Carbolante, ES; Covas, DT; Picanço-Castro, V. Forced expression of OCT4 influences the expression of pluripotent genes in human mesenchymal stem cells and fibroblasts.. Genetics and Molecular Research, v. 2, p. 1054-1060, 2013. 12. Pinto, MT; Malta, TM; Rodrigues, ES; Pinheiro, DG; Panepucci, RA; Panepucci, RA; Farias, KCRMD; De Paula Alves Sousa, A; Takayanagui, OM; Tanaka, Y; Covas, DT; Kashima, S. Genes related to antiviral activity, cell migration, and lysis are differentially expressed in CD4+ T cells in HAM/TSP patients.. AIDS Research and Human Retroviruses, v. 29, p. 130917035218000, 2013. 13. Brunetta, DM; De Santis, GC; Vilar, FC; Vilar, FC; Brandão, RA; Muniz, RZDA; De Lima, GMN; Amorelli-Chacel, ME; Covas, DT; Machado, AA. Hematological particularities and co-infections in injected drug users with AIDS. The Brazilian Journal of Infectious Diseases (Impresso), v. 13, p. S1413-8670(13)0, 2013. 14. Slavov, SN; Kashima, S; Silva-Pinto, AC; Amarilla, AA; Aquino, VH; Covas, DT. Molecular and clinical evaluation of the acute human parvovirus B19 infection: comparison of two cases in children with sickle cell disease and discussion of the literature. The Brazilian Journal of Infectious Diseases (Impresso), v. 17, p. 97-101, 2013. 15. De Santis, GC; Brunetta, DM; Nardo, M; Oliveira, LC; Souza, FF; Cagnolati, D; Mente, ÊD; Sankarankutty, AK; Covas, DT; De Castro E Silva, O. Preoperative variables associated with transfusion requirements in orthotopic liver transplantation. Transfusion and Apheresis Science, v. 1, p. 1, 2013. 16. Del Lama, LS; De Góes, EG; Petchevist, PCD; Moretto, EL; Borges, JC; Covas, DT; De Almeida, A. Prevention of Transfusion-Associated Graft-versus-Host Disease by Irradiation: Technical Aspect of a New Ferrous Sulphate Dosimetric System. Plos One, v. 8, p. e65334, 2013. 17. Rodrigues, ES; Kashima, S; Picanço-Castro, V; Espanhol, MR; De Andrade, LA; Palma, PV; Kashima, S; Fontes, AM; Covas, DT. Quantitative correlation between transcriptional levels of ER chaperone, peroximal protein and FVIII productivity in human Hek-293 cell line. SpringerPlus, v. 2, p. 328, 2013.