Wilson A Silva Jr
Full Professor of Genetics, FMRP/USP
1986-1989 Graduated in Biologic Sciences, Federal University of Pará, Brazil
|Research Interests||Cancer Genetics
|Research Overview||The major focus of our group is to understand the molecular mechanisms involved in genetic diseases and cancer development. We are using whole genomic analysis to identify and characterize genetic program/gene pathways that drive tumorigenic processes, and biomarkers of diagnosis, prognosis and therapeutic orientation. We are also interested in the characterization of biological properties of Cancer Stem Cells (CSCs), which present self-renewal capacity, unlimited proliferative potential, differentiation competence, and are extremely resistant to radio and chemotherapies. For this pourpose, we are applying next generation sequencing in order to profile gene expression, characterize epigenetic alterations, detect chromosomal rearrangements, and single nucleotide variations in coding and regulatory gene regions (RNAseq, ChIP-Seq, DNA methylation, Exome, and Paired-End protocols) in several solid tumors including: head and neck carcinoma, colorectal carcinoma, glioblastoma multiforme and osteosarcoma.|
|Lab Staff||Molecular Genetics Group
Júlio Lorenzi, PhD Student
Cleidson de Pádua Alves, Post-Doc/CNPq
Daniel Onofre Vidal, Post-Doc/FAPESP
Dalila Lucíola Zanette, Post-Doc/FAPESP
Molecular Biology Technicians
|Collaborators||Silvia Regina Caminada de Toledo, PhD Universidade Federal de São Paulo Laboratório de Genética firstname.lastname@example.org
Valeria Valente, PhD Faculdade de Ciências Farmacêuticas de Araraquara/UNESP Laboratório de Biologia Celular email@example.com
Houtan Noushmehr, PhD Faculdade de Medicina de Ribeirão Preto, USP Laboratório de Biologia Integrativa firstname.lastname@example.org
|International Collaborators||Gerhard A Coetzee, PhD Professor of Urology and Preventive Medicine USC/Norris Cancer Center, NOR6411 1441 Eastlake Ave Los Angeles, CA 90089
Gene Yeo, PhD Assistant Professor Department of Cellular and Molecular Medicine UCSD Bioinformatics Graduate Program USSD Stem Cell Initiative 9500 Gilman Drive, CMM-E La Jolla California, 92093-0695
|Facilities Coordination||DNA sequencing (ABI 3500 xL Genetic Analyzer)Next Generation Sequencing (Genome Analyzer IIx, Illumina)Bioinformatics Analysis|
|Selected Publications||1. Garcia DF, Oliveira TG, Molfetta GA, Garcia LV, Ferreira CA, Marques AA, Silva WA Jr. Biochemical and genetic analysis of butyrylcholinesterase (BChE) in a family, due to prolonged neuromuscular blockade after the use of succinylcholine. Genet Mol Biol. 2011 Jan;34(1):40-4
2. Montoro JR, Mamede RC, Neder Serafini L, Saggioro FP, Figueiredo DL, Silva WA Jr, Jungbluth AA, Spagnoli GC, Zago MA. Expression of cancer-testis antigens MAGE-A4 and MAGE-C1 in oral squamous cell carcinoma. Head Neck. 2011 Nov 15. doi: 10.1002/hed.21880.
3. Kannen V, Marini T, Zanette DL, Frajacomo FT, Silva GE, Silva WA Jr, Garcia SB. The melatonin action on stromal stem cells within pericryptal area in colon cancer model under constant light. Biochem Biophys Res Commun. 2011 Feb 25;405(4):593-8.
4. Silva IT, Vêncio RZ, Oliveira TY, Molfetta GA, Silva WA Jr. ProbFAST: Probabilistic functional analysis system tool. BMC Bioinformatics. 2010 Mar 30;11:161.
5. Pinheiro DG, Galante PA, de Souza SJ, Zago MA, Silva WA Jr. A score system for quality evaluation of RNA sequence tags: an improvement for gene expression profiling. BMC Bioinformatics. 2009 Jun 6;10:170.
6. Dos Santos ML, Gimenes KP, Silva WA Jr, Nagai MA. Transcriptome changes induced by docetaxel in human mammary cell lines expressing different levels of ERBB2. Int J Mol Med. 2009 Jun;23(6):733-43.
7. Silva WA Jr, Gnjatic S, Ritter E, Chua R, Cohen T, Hsu M, Jungbluth AA, Altorki NK, Chen YT, Old LJ, Simpson AJ, Caballero OL. PLAC1, a trophoblast-specific cell surface protein, is expressed in a range of human tumors and elicits spontaneous antibody responses. Cancer Immun. 2007 Nov 6;7:18.
8. Zanette DL, Rivadavia F, Molfetta GA, Barbuzano FG, Proto-Siqueira R, Silva-Jr WA, Falcão RP, Zago MA. miRNA expression profiles in chronic lymphocytic and acute lymphocytic leukemia. Braz J Med Biol Res. 2007 Nov;40(11):1435-40.
9. Amaral FC, Torres N, Saggioro F, Neder L, Machado HR, Silva WA Jr, Moreira AC, Castro M. MicroRNAs differentially expressed in ACTH-secreting pituitary tumors. J Clin Endocrinol Metab. 2009 Jan;94(1):320-3.10. Silva WA Jr, Covas DT, Panepucci RA, Proto-Siqueira R, Siufi JL, Zanette DL, Santos AR, Zago MA. The profile of gene expression of human marrow mesenchymal stem cells. Stem Cells. 2003;21(6):661-9.
Marco A. Zago
Full Professor of Hematology, FMRP/USP
|Education||1965-1970 Graduated in Medicine, Medical School of Ribeirão Preto, University of São Paulo, FMRP-USP, Brazil.
1971-1973 Master in Clinical Medicine, Medical School of Ribeirão Preto, University of São Paulo, FMRP-USP, Brazil.
1973-1975 PhD in Clinical Medicine, Medical School of Ribeirão Preto, University of São Paulo, FMRP-USP, Brazil.
1976-1977 Postdoctoral Research Fellow, Oxford University
1981 Full Professor, Medical School of Ribeirão Preto, University of São Paulo, FMRP-USP, Brazil
|Research Interests||Somatic and Pluripotent Stem Cells
Cancer and Leukemia
microRNA and Transcription Factors
|Research Overview||In the last few years, we have addressed the characterization of somatic stem cells, including Mesenchymal Stem Cells (MSC) and Hematopoietic Stem Cells (HSC) from different sources, to study the molecular basis of their specific biological characteristics, as well as to provide evidence for their distribution and in vivo role. In fact, we have helped to establish the concept that MSCs occupy a perivascular niche throughout the body, closely resembling pericytes, providing a cell repository for tissue repair and also playing important roles in the maintenance of peripheral immunehomeostasis. In that sense, we provided evidence of a new immunomodulatory mechanism exerted by MSC in response to inflammatory stimuli derived from activated T lymphocytes (Saldanha-Araujo et.al. 2011). Moreover, our results points to a role of MSCs in the modulation of NF-kB signaling in activated T lymphocytes, switching from a canonical pro-inflammatory to a non-canonical immunoregulatory role (Saldanha-Araujo et.al. 2011b). We have also provided evidence for the role of non-canonical NF-kB signaling in the characteristic molecular features of HSC derived from Umbilical Cord Blood (UCB), as compared to Bone Marrow (BM). We are currently starting to address the molecular mechanisms by which MSC become activated to exert their immunomodulatory properties, upon treatment with pro-inflammatory stimuli (a mechanism generally referred as MSC “licensing” or “conditioning”). Finally, we are currently using synthetic pre-miRs and anti-miRs to explore the roles of microRNAs in the regulation of distinct stem cell characteristics (including pluripotency, differentiation, self-renewal, among others), and to identify functionally-relevant microRNA targets (trough coupled microarray profiling).|
Molecular Biology Technicians
Vanderson Rocha, PhD, MD (Hosp. Sírio Libanês, SP).
Felipe Saldanha Araujo, PhD (UNB, Brasília-DF).
Francisco de Paula Careta, PhD (UFES, -ES).
|Facilities Coordination||Agilent 48-slides auto-loader High-Density Microarray Scanner and Axon GenePix 4000B (Molecular Devices).|
1. Saldanha-Araujo F., Haddad R., Malmegrim de Farias K.C., Alves Souza A.D., Palma P.V., Araujo A.G., Orellana M.D., Voltarelli J.C., Covas D.T., Zago M.A., Panepucci RA Mesenchymal stem cells promote the sustained expression of CD69 on activated T-lymphocytes: roles of canonical and non-canonical NF-kappaB signaling. Journal of cellular and molecular medicine. 2011a Jul 21.