CV

Lygia V. Pereira

Full Professor of Genetics, IB-USP
Head of the National Laboratory of Embryonic Stem Cells (LaNCE)

lpereira@usp.br
PubMed

1984-1989 Bachelor in Physiscs, Pontifícia Universidade Católica, Rio de Janeiro, RJ.
1989-1990 M.S. in Molecular Biology, Instituto de Biofísica Carlos Chagas Filho, UFRJ, Rio de Janeiro, RJ.
1989-1994 PhD in Human Molecular Genetics, City University of New York, Mount Sinai Graduate School, New York, NY, USA.
2004 Full Professor, University of São Paulo, Brazil.

Human Molecular Genetics
Marfan Syndrome
Pluripotent Stem Cells
Epigenetics – X chromosome inactivation

(1) Marfan Syndrome (MFS) – lessons from animal models: MFS is an autosomal dominant disease of the connective tissue that affects the ocular, skeletal and cardiovascular systems. The disease is caused by mutations in the FBN1 gene, that code the fibrillin-1 protein, a major structural component of the extracellular microfibers. By homologous recombination in ES cells, we have established two mouse models for MFS: one containing a FBN1 null allele (mgNull), and another with a deletion of exons 18-25 (mgΔloxPneo). In these models we study the mechanisms of pathogenesis of the disease; genetic and epigenetic factors modifying the phenotype, and we try new therapies for MFS.
(2) Epigenetic inheritance – X chromosome inactivation (XCI): we are particularly interested in the process of maintenance of the inactive state of the X chromosome throughout mitosis in human cells. For that, we have developed functional tests that allow the screening of new genes involved in this process. We also work in the characterization of human embryonic stem cells as an in vitro model for the study of XCI.
(3) Establishment of new lines of human embryonic stem cells (hESC): Our group is involved in the derivation of new lines of hESC from the Brazilian population, for use in research and therapy. Our interest is to study the epigenetic state of these cells cultured/derived in different conditions.
(4) Analysis of the role of GBA mutations in the development of Parkinsonism using induced pluripotent stem cells (iPSC): Recent works have shown a higher frequency of Gaucher disease causing GBA mutations in patients with Parkinsonism (PD). This projects aims the establishment of iPSC lines from patients with PD and mutations in the GBA gene, where we will be able to study the molecular mechanisms behind this association.
(5) National Laboratory of Emrbyonic Stem Cells (LaNCE): this is one of eight Centers for Cell Technology sponsored by the Minsitries of Health, and Science and Technology for the production of stem cells under GMP conditions. LaNCE’s specific mission is to promote research and therapy with pluripotetn stem cells in Brazil. This is a project in collaboration with Prof. Stevens Rehen’s group, at UFRJ.

Molecular Genetics Group
Erica Sara M.S. , Student/FAPESP
Fernando Sábio M.S., Student/FAPESP
Gustavo Ribeiro Fernandes, PhD Student/FAPESP
Fernanda Silvestre, PhD Student/CAPES
Ana Maria Fraga, PhD Student/FAPESP
Cynthia Quinderé Cardoso, PhD Student/CNPq
Joana Carvalho Moreira de Mello, PhD Student/FAPESP
Naja Vergani, PhD Student/CAPES
Giovana Pirolla Cardozo, PhD Student/FAPESP
Simone Fonseca, Post-Doc/CAPES
Bruno Lazzari Lima, Post-Doc/FAPESP
Mariana Morato Marques, Technical support
Ana Patrícia Kamisaki Santana, Administrative support

Egberto Reis Barbosa, PhD
Universidade de São Paulo, Faculdade de Medicina da Universidade de São Paulo, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo. 
Av. Dr. Enéas de Carvalho Aguiar, 255
Cerqueira César
05403-000 – Sao Paulo, SP – Brasil
Telefone: (11) 30696401 Fax: (11) 30827548
http://www.fm.usp.br/

Silvia Maria Gomes Massironi, PhD
Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Imunologia.
AV. LINEU PRESTES 1730
CIDADE UNIVERSITARIA
05508-900 – Sao Paulo, SP – Brasil
Telefone: (11) 30917398 Fax: (11) 30917224

Jeanne Loring, PhD
Professor of Developmental Neurobiology
Director of the Center for Regenerative Medicine
Department of Chemical Physiology
California Campus
Laboratory Website
jloring@scripps.edu
(858) 784-7767 

FRAGA AM, ARAÚJO ESS, STABELLINI R, VERGANI N, PEREIRA LV. A Survey of Parameters Involved in the Establishment of New Lines of Human Embryonic Stem Cells. Stem Cell Reviews, v.1, p.1 – , 2011.AMPS K, ANDREWS PW, ANYFANTIS G, ARMSTRONG L, AVERY S, BAHARVAND H, BAKER J, BAKER D, MUNOZ MB, BEIL S, BENVENISTY N, BEN-YOSEF D, BIANCOTTI JC, BOSMAN A, BRENA RM, BRISON D, CAISANDER G, CAMARASA MV, CHEN J, CHIAO E, CHOI YM, CHOO ABH, COLLINS D, COLMAN A, CROOK J M, DALEY GQ, DALTON A, DE SOUSA PA, DENNING C, DOWNIE J, DVORAK, MONTGOMERY KD, FEKI A, FORD A, FOX V, FRAGA AM, FRUMKIN T, GE L, GOKHALE PJ, GOLAN-LEV T, PEREIRA, LV. et al. Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. Nature Biotechnology, 29:1132–1144, 2011.Fraga, A.M. ; Soukoyan, M. ; Rajan, P ; de Almeida Ferreira Braga, Daniela ; IACONELLI JR., A. ; José Gonçalves Franco Jr ; BORGES JR., E. ; Pereira, L.V. . Establishment Of A Brazilian Line Of Human Embryonic Stem Cells In Defined Medium – Implications For Cell Therapy In An Ethnically Diverse Population. Cell Transplantation, v. 1, p. 1, 2010.Moreira de Mello, Joana Carvalho ; Araújo, Érica Sara Souza de ; Stabellini, Raquel ; Fraga, Ana Maria ; Souza, Jorge Estefano Santana de ; Sumita, Denilce R. ; Camargo, Anamaria A. ; Pereira, Lygia V. . Random X Inactivation and Extensive Mosaicism in Human Placenta Revealed by Analysis of Allele-Specific Gene Expression along the X Chromosome. Plos One, v. 5, p. e10947, 2010.E. Sidransky, et al. Multicenter Analysis of Glucocerebrosidase Mutations in Parkinson’s Disease. New England Journal of Medicine, 361:1651-61, 2009.Raquel Stabellini, Joana Carvalho Moreira de Mello, Lys Molina Hernandes, Lygia V. Pereira. MAOA and GYG2 are submitted to X chromosome inactivation in human fibroblasts. Epigenetics, 4:1-8, 2009.VASQUES, L.R., STABELINI, R., SUKOYAN, M., E PEREIRA, L.V. XIST repression in the absence of DNMT1 and DNMT3B. DNA Research, 12:373-378, 2005.CARTA C, PEREIRA L, ARTEAGA-SOLIS E, LEE-ARTEAGA SY, STARCHER B, MERKEL CA, SUKOYAN M, KERKIS A, HAZEKI N, KEENE DR, SAKAI LY E RAMIREZ F. Fibrillins 1 and 2 perform partially overlapping roles during aortic development. Journal of Biological Chemistry 281(12):8016-8023, 2005.XUE, F., TIAN, C., DU, F., KUBOTA, C., TANEJA, M., DINNYES, A., DAI, Y., LEVINE, H., PEREIRA, L.V., E YANG, X. Aberrant patterns of X chromosome inactivation in bovine clones. Nature Genetics, vol. 31(6):216-220, 2002.Sukoyan, M.A., Kerkis, A.Y., Mello, M.R.B., Kerkis, I.E., Visintin, J.A., Pereira, L.V. Establishment of new murine embryonic stem (ES) cell lines for the generation of mouse models for human genetic diseases. Braz. J. Med. Biol. Res., vol. 35(5):535-542, 2002.Vasques, L.R., and Pereira, L.V. Allele-specific X-linked gene activity in normal human cells assayed by expressed single nucleotide polymorphisms (cSNPs). DNA Research, vol. 8(4):173-177, 2001.

Lewis Joel Greene

Full Professor of Cell and Molecular Biology (retired) FMRP/USP
Supervisor of the Center for Protein Chemistry, at Regional Blood Center of Ribeirão Preto 
Volunteer head teacher (senior associate) of FMRP/USP

ljgreene@fmrp.usp.br
PubMed Scopus  ResearchID  Scielo Scholar

1951-1955 B.A (magna cum laude), Amherst College, Amherst Massachusetts
1955-1962 Ph D, Rockefeller University, C.H.W. HIRS and G.E. Palade
(Research advisors)
1990 Full Professor, University of São Paulo, Brazil

Carolina Hassibe Thomé, Pos Doc
Germano Aguiar Ferreira, Doctoral Student

José César Rosa
Faculdade de Medicina de Ribeirão Preto – Universidade de São Paulo
Centro de Química de Proteínas, Hemocentro de Ribeirão Preto, SP
jcrosa@fmrp.usp.br

Andréia Machado Leopoldino
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo
andreiaml@usp.br

Dimas Tadeu Covas
Faculdade de Medicina de Ribeirão Preto – Universidade de São Paulo
Centro Regional de Hemoterapia de Ribeirão Preto
dimas@fmrp.usp.br

Vitor Marcel Faça
Prof. Dr. da Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo
Department of Biochemistry and Immunology
vitor.faca@fmrp.usp.br

Eduardo Magalhães Rego
Departamento de Clínica Médica da Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo
edumrego@hotmail.com

Wilson Araújo da Silva Júnior
Departamento de Genética da Faculdade de Medicina de Ribeirão Preto – Universidade de São Paulo
wilsonjr@usp.br

Roger Chammas
Professor Titular, Faculdade de Medicina, Universidade de São Paulo – USP
Departamento de Radiologia
rchammas@lim24.fm.usp.br

1. SOBRAL, L. M. ; SOUSA, L.O. ; COLETTA, R. D. ; CABRAL, H. ; GREENE, L. J. ; TAJARA, E.H. ; GUTKIND, J. S. ; CURTI, C. ; Leopoldino, A. M. . Stable SET knockdown in head and neck squamous cell carcinoma promotes cell invasion and the mesenchymal-like phenotype in vitro, as well as necrosis, cisplatin sensitivity and lymph node metastasis in xenograft tumor models. Molecular Cancer , v. 13, p. 32, 2014.

2. Thomé, C. H. ; DOS SANTOS, G. A. ; FERREIRA, G. A. ; SCHEUCHER, P. S. ; IZUMI, C. ; Leopoldino, A. M. ; SIMAO, A. M. ; CIANCAGLINI, P. ; DE OLIVEIRA, K. T. ; CHIN, A. ; HANASH, S. M. ; FALCAO, R. P. ; REGO, E. M. ; Greene, L. J. ; Faça, V. M. . Linker for Activation of T-cell Family Member2 (LAT2) a Lipid Raft Adaptor Protein for AKT Signaling, Is an Early Mediator of Alkylphospholipid Anti-leukemic Activity. Molecular & Cellular Proteomics , v. 11, p. 1898-1912, 2012. Citações: 3| 4

3. Fontes, A. M. ; Melo, F.U.F. ; Greene, L.J. ; Faça, V. M. ; Lin, Y. ; Gerson, S.L. ; Covas, D. T. . Production of human factor VIII-FL in 293T cells using the bicistronic MGMT(P140K)-retroviral vector. Genetics and Molecular Research , v. 11, p. 775-789, 2012.

4. Miranda, H. C. ; HERAI, R. H. ; Thomé, C. H. ; Gomes, G. G. ; PANEPUCCI, R. A. ; ORELLANA, M. D. ; Covas, D. T. ; Muotri, A. R. ; Greene, L.J. ; Faça, V. M. . A quantitative proteomic and transcriptomic comparison of human mesenchymal stem cells from bone marrow and umbilical cord vein. Proteomics (Weinheim. Print) , v. 12, p. 2607-2617, 2012. Citações: 3| 6

5. Spiller, F. ; FERREIRA, S. H. ; Greene, L. J. ; Rosa, J. C. ; Altruda, F. ; Souto, F. O. ; Hirsch, E. ; Mestriner, F. L. A. C. ; Cunha, F. Q. ; Tolosano, E. ; Alves-Filho, J. C. ; Laure, H. J. ; Freitas, A. ; Costa, C. ; Vinchi, F. . Inhibition of Neutrophil Migration by Hemopexin Leads to Increased Mortality Due to Sepsis in Mice. American Journal of Respiratory and Critical Care Medicine , v. 183, p. 922-931, 2011. Citações: 7| 7

6. Santos, G. A. S. ; Thomé, C. H. ; FERREIRA, G.A. ; YONEDA, J. S. ; NOBRE, T. M. ; Daghastnali, K.R.P. ; SCHEUCHER, P. S. ; TEIXEIRA, H. L. ; Constantino, M. G. ; DE OLIVEIRA, K. T. ; Faça, V. M. ; FALCAO, R. P. ; GREENE, L. J. ; REGO, E. M. ; CIANCAGLINI, P. . Interaction of 10-(octyloxy) decyl-2-(trimethylammonium) ethyl phosphate with mimetic membranes and cytotoxic effect on leukemic cells. Biochimica et Biophysica Acta. Biomembranes , v. 1798, p. 1714-1723, 2010. Citações: 4| 5

7. Mestriner, F. L. A. C. ; Spiller, F. ; Laure, H. J. ; Souto, F. O. ; TAVARES-MURTA, B.M. ; Rosa, J. C. ; Basile Filho, A. ; FERREIRA, S. H. ; GREENE, L. J. ; Cunha, F. Q. . Acute-phase protein α -1-acid glycoprotein mediates neutrophil migration failure in sepsis by a nitric oxide-dependent mechanism.. Proceedings of the National Academy of Sciences of the United States of America (Online) , v. 104, p. 19595-19600, 2007. Citações: 20| 22

8. SOUZA, G. A. ; GODOY, L.M.F. de ; TEIXEIRA, V. R. ; OTAKE, A. H. ; SABINO, A. ; Rosa, J. C. ; Dinarte, A. R. ; Pinheiro, D. G. ; Silva Jr, W. A. ; EBERLIN, M. N. ; Chammas, R. ;GREENE, L. J. . Proteomic and SAGE profiling of murine melanoma progression indicates the reduction of proteins responsible for ROS degradation. Proteomics, Inglaterra, v. 6, n.5, p. 1460-1470, 2006. Citações: 22| 22

Flávio V. Meirelles

Full Professor of Cell Biology & Development, FZEA/USP
Member of the Coordinating Committee (Veterinary Medicine) of FAPESP
Associate editor of Genetics And Molecular Research

meirellf@usp.br
PubMed Scopus ORCID  ResearcherID Scholar 

1989-1993 Graduated in Veterinary Medicine, São Paulo State University, Brazil
1994-1996 Master Thesis in Reproduction, University of Montreal, Canada
1997-1999 PhD in Genetics, Medical Scholl of Ribeirão Preto, University of  São Paulo
2004 Full Professor, University of São Paulo, USP, Brazil

Cytoplasmic inheritance
Nuclear Reprograming
Animal Genetics
Mitochondrial genome
Embryonic genome activation

Pos Docs
Marcos Roberto Chiaratti, PhD
Lilian de Jesus Oliveira,PhD
José Rodrigo V. Pimentel, PhD

PhD
Fabiana Fernandes Bressan,MSc
Reno Roldi Araujo, MSc
Rodrigo Barreto, MSc
Rafael Sampaio, MSc
Juliano Sangalli, MSc
Pedro Ratto Pires, MSc

MSc
Lais Vicari Pessoa, DVM
Gabriella Mamede Andrade, DVM

Claudia Lima Verde Leal, PhD
Universidade Federal de São Paulo
LMMD

Carlos Eduardo Ambrósio, PhD
Universidade Federal de São Paulo
LMMD

Felipe Perecin, PhD
Universidade Federal de São Paulo
LMMD

Heidge Fukumasu
Universidade Federal de São Paulo
LMMD

Maria Angélica Miglino
Universidade Federal de São Paulo
FMVZ – Programa de Pós Graduação em Anatomia

Joanna Poulton, PhD
Professor of Genetics
Univ. of Oxford,

Lawrence Charles Smith, PhD
Full Professor – Canadian Chair of Animal Cloning
Université de Montréal

Willian Allan King
Full Professor – Canadian Chair of Animal Biotechnology
Guelph – ON Canada

1. BRESSAN, Fabiana Fernandes ; Dos Santos Miranda, Moyses ; PERECIN, Felipe ; De Bem, Tiago Henrique ; Pereira, Flavia Thomaz Verechia ; Russo-Carbolante, Elisa Maria ; Alves, Daiani ; Strauss, Bryan ; Bajgelman, Marcio ; Krieger, José Eduardo ; Binelli, Mario ; Meirelles, Flavio Vieira . Improved Production of Genetically Modified Fetuses with Homogeneous Transgene Expression After Transgene Integration Site Analysis and Recloning in Cattle. Cellular Reprogramming (Formerly Cloning and Stem Cells), v.13 (1) p.29-36, 2011.
2. CHIARATTI, Marcos Roberto; FERREIRA, Christina Ramires; PERECIN, Felipe … Meirelles, Flávio Vieira . Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. Reprod. BioMedicine Online, v. 22, p. 172-183, 2011.
3. Suzuki Jr, João ; Therrien, Jacinthe ; Filion, France ; Lefebvre, Rejean …Perecin, Felipe ; Meirelles, Flávio V. ; Smith, Lawrence Charles . Loss of Methylation at H19 DMD Is Associated with Biallelic Expression and Reduced Development in Cattle Derived by Somatic Cell Nuclear Transfer. Biology of Reproduction, v. 84, p. 947-956, 2011.
4. Bressan, Fabiana Fernandes; Perecin, Felipe; Sangalli, Juliano Rodrigues ; Meirelles, F. V. Reprogramming somatic cells: pluripotency through gene induction and nuclear transfer. Acta Scientiae Veterinariae, v. 39, p. s83-s95, 2011.
5. De Bem, T. H. C. ; Chiaratti, M. R. ; Rochetti, R. ; Bressan, F. F. ; Sangalli, J. R. ; Miranda, M. S. ; Pires, P. R. L. ; Schwarz, K.R.L. ; Sampaio, R. V. ; Fantinato Neto, P. ; Pimentel, J. R. V. ; Perecin, F. ; Smith, L. C. ; Meirelles, F. V. ; Leal, C. L. V. . Viable calves produced by somatic nuclear transfer using meiotic-blocked oocytes. Cellular reprogramming, v. 13, p. DOI: 10.1089/c, 2011.
6. Chiaratti, M R ; Meirelles, F. V. ; Wells, D ; Poulton, J . Therapeutic treatments of mtDNA diseases at the earliest stages of human development. Mitochondrion (Amsterdam. Print), v. 11, p. 820-828, 2011.
7. Ferreira, C. R. ; Burgstaller, J. P. ; Perecin, F. ; Garcia, J. M. ; Chiaratti, M. R. ; Meo, S. C. ; Muller, M. ; Smith, L. C. ; Meirelles, F. V. Pronounced Segregation of Donor Mitochondria Introduced by Bovine Ooplasmic Transfer to the Female Germ-Line. Biology of Reproduction, v. 82, p. 563-571, 2010.
8. Meirelles, Flávio V. ; Birgel, Eduardo H. ; PERECIN, Felipe Et Al., Delivery of cloned offspring: experience in Zebu cattle. Reprod., Fertility and Dev. v. 22, p. 88, 2010.
9. Chiaratti, M. R. ; Meirelles, F. V. . Mitochondrial DNA Copy Number, a Marker of Viability for Oocytes. Biology of Reproduction, v. 83, p. 1-2, 2010.
10. Ferreira, Christina Ramires ; Saraiva, Sérgio Adriano ; Catharino, Rodrigo Ramos ; Garcia, Jerusa Simone ; Gozzo, Fábio César ; Sanvido, Gustavo Braga ; Santos, Luiz Fernando Arruda ; Turco, Edson Guimarães Lo ; Pontes, José Henrique Fortes ; Basso, Andréa Cristina ; Bertolla, Ricardo Pimenta ; Sartori, Roberto ; Guardieirio, Monique Mendes ; Perecin, Felipe ; Meirelles, F. V; Eberlin, Marcos Nogueira . Single embryo and oocyte lipid fingerprinting by mass spectrometry. Journal of Lipid Research (Print), v. 51, p. 1218-1227, 2010.
11. Chiaratti, Marcos Roberto ; Ferreira, Christina Ramires ; Meirelles, Flávio Vieira ; et al. Xenooplasmic Transfer between Buffalo and Bovine Enables Development of Homoplasmic Offspring. Cellular Reprogramming (Formerly “Cloning and Stem Cells”), v. 12, p. 231-236, 2010.
14. Poulton, Joanna ; Chiaratti, Marcos R. ; Meirelles, Flávio V. ; Kennedy, Stephen ; Wells, Dagan ; Holt, Ian J. ; Suomalainen, Anu . Transmission of Mitochondrial DNA Diseases and Ways to Prevent Them. PLoS Genetics, v. 6, p. e1001066-08, 2010.
15. Perecin, F. ; Meo, S.C. ; Yamazaki, W. ; Ferreira, C.R. ; Merighe, G.K.F. ; Meirelles, F.V. ; Garcia, J.M. . Imprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes. Gen. and Mol. Res., v. 8, p. 76-85, 2009.
16. Dos Santos Miranda, Moysés ; BRESSAN, Fabiana Fernandes ; Zecchin, Karina Gottardello ; Vercesi, Anibal Eugênio ; MESQUITA, Ligia Garcia ; Fonseca Merighe, Giovana Krempel ; KING, William Allan ; Ohashi, Otávio Mitio ; Pimentel, José Rodrigo Valim ; PERECIN, Felipe ; Meirelles, Flávio Vieira . Serum-Starved Apoptotic Fibroblasts Reduce Blastocyst Production but Enable Development to Term after SCNT in Cattle. Cloning and Stem Cells, v. 11, p. 1-9, 2009.
17 Chiaratti, M. R. ; Bressan, F. F. ; Ferreira, C. R. ; Caetano, A. R. ; Smith, L. C. ; Vercesi, A. E. ; Meirelles, F. V. . Embryo Mitochondrial DNA Depletion Is Reversed During Early Embryogenesis in Cattle. Biology of Reproduction, v. 82, p. 76-85, 2009.
18. Ferreira, E.M. ; Vireque, A.A. ; Adona, P.R. ; Meirelles, F.V. et al., Cytoplasmic maturation of bovine oocytes: Structural and biochemical modifications and acquisition of developmental competence. Theriogenology, v. 71, p. 836-848, 2009.
19. BIASE, Fernndo Henrique ; MARTELLI, L ; MERIGHE, Giovana Krempel Fonseca ; BIASE, Weruska Karyna Freitas Santos ; MIRANDA, Moysés Dos Santos ; SMITH, L. C. ; Meirelles, F. V.A retrospective model of oocyte competence: global mRNA and housekeeping transcripts are not associated with in vitro developmental outcome.. Zygote (Cambridge), v. 17, p. 289-295, 2009.
20. Bressan, F.F. ; De Bem, T.H.C. ; Perecin, F. ; Lopes, F.L. ; Ambrosio, C.E. ; Meirelles, F.V. ; Miglino, M.A. . Unearthing the Roles of Imprinted Genes in the Placenta. Placenta (Eastbourne), v. 30, p. 823-834, 2009.

Eduardo Magalhães Rego

Professor of Hematology/Oncology, Medical School of Ribeirão Preto, University of São Paulo
Head of the Hematology and Clinical Oncology Service of the University Hospital (HC-FMRP)
Coordinator in Brazil of the International Consortium on Acute Leukemias which is sponsored by the American Society of Hematology
Member of the Board of Directors of the Brazilian Association of Hematology (ABHH)
Editor of the Brazilian Journal of Medical and Biological Research and Associate
Editor of Blood and Annals of Hematology

emrego@hcrp.fmrp.usp.br
PubMed Scopus ORCID  ResearcherID Scholar 

1983-1988 Doctor of Medicine (M.D.) Medical School of Ribeirão Preto of the University of São Paulo
1989-1992 Medical Residence/ Fellowship in Hematology, University Hospital, Medical School of Ribeirão Preto of the University of São Paulo
1993-1997 Doctor of Philosophy (Ph.D.) Medical School of Ribeirão Preto of the University of São Paulo
1998-2001 Post-Doctoral Fellowship Memorial Sloan-Kettering Cancer Center, New York, USA
2002 Full Professor, University of São Paulo, USP, Brazil
2005 PhD in Medicine, Fox Chase Cancer Center, FCCC, USA

Hematological malignancies, in particular acute leukemias
Leukemogenesis
Animal models of acute leukemia
Leukemia and cancer stem cells

BV-CDI FAPESP

The main focus of our group is to integrate basic and clinical research on acute leukemias, in special acute myeloid leukemias. In order to have a better insight on the molecular basis of leukemogenesis, we have been studying acute promyelocytic leukemia (APL) as a model. We have been addressing the following questions:

1. Which cell subpopulation acts the leukemia initiating cells and how is quiescence and energy generation regulated in this subset;

2. Which microRNAs act as key regulators of differentiation in APL blasts and whether their expression profile are associated with treatment outcome;

3. Does the expression of transcriptinally active and inactive isoforms of the p73 gene affect the response to ATRA and if so how?

4. What is the role of microparticles and annexin II expression in APL-associated coagulopathy;

5. Can the molecular monitoring of the PML/RARA fusion gene using RQ-PCR technology predict relapse better than the routinely used RT-PCR method?;

6. Is leukemia relapse is associated with additional mutagenic events?

7. In leukemia prone syndromes, such as dyskeratosis congenita, which pathways confer survival advantage to hematopoietic progenitors and are associated in malignant transformation. Finally, our group is coordinating in Brazil the International Consortium on APL, which is a trial aiming to improve the treatment outcome of APL patients in developing countries.

1. PhD students:
a. Ana Paula A. de L. Lange
b. Antonio R. L. de Araújo
c. Helder Henrique Paiva
d. Sarah Cristina Bassi
2. MsC students:
a. Katarina Holanda
3. Post-docs:
a. Priscila Santos Scheucher
b. Guilherme Augusto Silva dos Santos

Molecular Biology Technicians
Ana Sílvia Gouveia Lima

Andréia M Leopoldino, PhD
Depto de Análises Clínicas, Toxicológicas e Bromatológicas
Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo

Dr Davide Rugero,
Associate Professor
Helen Diller Family
Cancer Research Center
University of California,
San Francisco (UCSF)
Mission Bay Campus
1450 3rd Street
Room HD 386, MC 3110
San Francisco, CA 94158
Tel: 415/514-9755
Fax: 415/514-4826
davide.ruggero@ucsf.edu

Prof. Ellen Solomon,
Prof. David Grimwade,
King’s College London
School of Medicine
Department of Medical and Molecular Medicine
Floor 8 Tower Wing
Guy’s Hospital
London SE1 9RT
Telephone : 0207 1882579
david.grimwade@genetics.kcl.ac.uk

http://www.kcl.ac.uk/medicine/research/divisions/gmm/sections/clusters/cancer/solomon/index.aspx
http://www.kcl.ac.uk/medicine/research/divisions/gmm/sections/clusters/cancer/grimwade/pgrimwade.aspx

Prof. Hau C.Kwaan,
Marjorie C. Barnett
Professor in Hematology-Oncology
Professor of Medicine
Northwestern University Feinberg School of Medicine
Room 8258, Olson Pavilion
710 N.Fairbanks Court,
Chicago, IL. 60611
h-kwaan@northwestern.edu
http://fsmweb.northwestern.edu/faculty/FacultyProfile.cfm?xid=14961

Prof. Stefan Bohlander,
Department of Medicine III,
Universität München,
81377 Munich, Germany
bohlander@helmholtz-muenchen.de
http://www.sfb684.med.uni-muenchen.de/projects/a6bohlander/index.html

Flow Cytometry and Sorting Facility,
And Small Animals Irradiator Facility,
Medical School of Ribeirão Preto,
University of São Paulo,
FAPESP EMU – grant 09/54218-1

1. Benicio MTL et al. Evaluation of the European LeukemiaNet recommendations for predicting outcomes of patients with acute myeloid leukemia treated in low- and middle-income countries (LMIC): A Brazilian experience. Leuk Res. 2017; 60:109-114..
2. Corrêa de Araujo Koury L et al. Treating acute promyelocytic leukaemia in Latin America: lessons from the International Consortium on Acute Leukaemia experience. Br J Haematol. 2017;177(6):979-983.
3. Bittencourt R et al. Guidelines on the treatment of acute myeloid leukemia: Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular: Project guidelines: Associação Médica Brasileira – 2015. Rev Bras Hematol Hemoter. 2016; 38(1):58-74.
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