Eduardo Vidal

Marco Antonio Zago

		Marco A. Zago Full Professor of Hematology, FMRP/USP Member of the Brazilian Academy of Sciences Dean of the University of São Paulo Former President of CNPq (National Council for Scientific and Technological Development) Former President and scientific director of the Regional Blood Center of Ribeirão Preto Clinical director of the University Hospital of Medical School of Ribeirão Preto marazago@usp.br PubMed ResearcherID Scholar Scopus View the complete Lattes Curriculum Vitae
Education		1965-1970 Graduated in Medicine, Medical School of Ribeirão Preto, University of São Paulo, FMRP-USP, Brazil. 1971-1973 Master in Clinical Medicine, Medical School of Ribeirão Preto, University of São Paulo, FMRP-USP, Brazil. 1973-1975 PhD in Clinical Medicine, Medical School of Ribeirão Preto, University of São Paulo, FMRP-USP, Brazil. 1976-1977 Postdoctoral Research Fellow, Oxford University 1981 Full Professor, Medical School of Ribeirão Preto, University of São Paulo, FMRP-USP, Brazil
Research Interests		Somatic and Pluripotent Stem Cells Cancer and Leukemia Functional Genomics microRNA and Transcription Factors
Research Overview		In the last few years, we have addressed the characterization of somatic stem cells, including Mesenchymal Stem Cells (MSC) and Hematopoietic Stem Cells (HSC) from different sources, to study the molecular basis of their specific biological characteristics, as well as to provide evidence for their distribution and in vivo role. In fact, we have helped to establish the concept that MSCs occupy a perivascular niche throughout the body, closely resembling pericytes, providing a cell repository for tissue repair and also playing important roles in the maintenance of peripheral immunehomeostasis. In that sense, we provided evidence of a new immunomodulatory mechanism exerted by MSC in response to inflammatory stimuli derived from activated T lymphocytes (Saldanha-Araujo et.al. 2011). Moreover, our results points to a role of MSCs in the modulation of NF-kB signaling in activated T lymphocytes, switching from a canonical pro-inflammatory to a non-canonical immunoregulatory role (Saldanha-Araujo et.al. 2011b). We have also provided evidence for the role of non-canonical NF-kB signaling in the characteristic molecular features of HSC derived from Umbilical Cord Blood (UCB), as compared to Bone Marrow (BM). We are currently starting to address the molecular mechanisms by which MSC become activated to exert their immunomodulatory properties, upon treatment with pro-inflammatory stimuli (a mechanism generally referred as MSC “licensing” or “conditioning”). Finally, we are currently using synthetic pre-miRs and anti-miRs to explore the roles of microRNAs in the regulation of distinct stem cell characteristics (including pluripotency, differentiation, self-renewal, among others), and to identify functionally-relevant microRNA targets (trough coupled microarray profiling).
Lab Staff		Laboratory Coordinator Prof. Rodrigo A. Panepucci, PhD Researcher (FUNDHERP) Fellows Researchers Rodrigo Haddad, PhD Post-Doc/FAPESP Lucila Habib Bourguignon Oliveira PhD student/FAPESP Mariane Serra Fraguas PhD student/FAPESP Danuta Sastre PhD student/CAPES Ildercílio Mota de Souza Lima Ms student/CAPES Lara Martinelli Zapata Ms student/CAPES Bruno Braga Sangiorgi Ms student/CAPES Vitor Leão Ms student/CAPES Molecular Biology Technicians Amélia Góes Araujo (FMRP-USP) Marli Haydee Tavella (FUNDHERP) Josiane Lilian dos Santos Schiavinato, Ms. (FMRP-USP)
Collaborators		Vanderson Rocha, PhD, MD (Hosp. Sírio Libanês, SP). Hosp. Sírio Libanês, SP silviatoledo@graacc.org.br Felipe Saldanha Araujo, PhD (UNB, Brasília-DF). UNB, Brasília-DF. silviatoledo@graacc.org.br Francisco de Paula Careta, PhD (UFES, -ES). UFES, -ES. silviatoledo@graacc.org.br
International Collaborators		Jaques Elion Inserm, UMR 763, Hôpital Robert Debré, Université Paris Diderot, France. Eliane Gluckman Eurocord, EBMT, Hôpital St. Louis Hospital, France. Carlos Martinez-A Centro Nacional de Biotecnología – CSIC, Univ. Autónoma de Madrid, Espanha Stephen Spellman CIBMTR National Marrow Donor Program, Minneapolis.
Facilities Coordination		Agilent 48-slides auto-loader High-Density Microarray Scanner and Axon GenePix 4000B (Molecular Devices).
Selected Publications		1. Saldanha-Araujo F., Haddad R., Malmegrim de Farias K.C., Alves Souza A.D., Palma P.V., Araujo A.G., Orellana M.D., Voltarelli J.C., Covas D.T., Zago M.A., Panepucci RA Mesenchymal stem cells promote the sustained expression of CD69 on activated T-lymphocytes: roles of canonical and non-canonical NF-kappaB signaling. Journal of cellular and molecular medicine. 2011a Jul 21. 2. Saldanha-Araujo F., Ferreira F.I., Palma P.V., Araujo A.G., Queiroz R.H., Covas D.T., Zago M.A., Panepucci RA Mesenchymal stromal cells up-regulate CD39 and increase adenosine production to suppress activated T-lymphocytes. Stem cell research. 2011b Jul;7(1):66-74. 3. Picanco-Castro V., Russo-Carbolante E., Reis L.C., Fraga A.M., de Magalhaes D.A., Orellana M.D., Panepucci RA, Pereira L.V., Covas D.T. Pluripotent reprogramming of fibroblasts by lentiviral mediated insertion of SOX2, C-MYC, and TCL-1A. Stem cells and development. 2011 Jan;20(1):169-80. 4. De Molfetta G.A., Luciola Zanette D., Alexandre Panepucci R., Dos Santos A.R., da Silva W.A., Jr., Antonio Zago M. Role of NFKB2 on the early myeloid differentiation of CD34+ hematopoietic stem/progenitor cells. Differentiation; research in biological diversity. 2010 Nov-Dec;80(4-5):195-203. 5. Panepucci RA, Oliveira L.H., Zanette D.L., Viu Carrara Rde C., Araujo A.G., Orellana M.D., Bonini de Palma P.V., Menezes C.C., Covas D.T., Zago M.A. Increased levels of NOTCH1, NF-kappaB, and other interconnected transcription factors characterize primitive sets of hematopoietic stem cells. Stem cells and development. 2010 Mar;19(3):321-32. 6. Covas D.T., Panepucci RA, Fontes A.M., Silva W.A., Jr., Orellana M.D., Freitas M.C., Neder L., Santos A.R., Peres L.C., Jamur M.C., Zago M.A. Multipotent mesenchymal stromal cells obtained from diverse human tissues share functional properties and gene-expression profile with CD146+ perivascular cells and fibroblasts. Experimental hematology. 2008 May;36(5):642-54. 7. Proto-Siqueira R., Panepucci RA, Careta F.P., Lee A., Clear A., Morris K., Owen C., Rizzatti E.G., Silva W.A., Jr., Falcao R.P., Zago M.A., Gribben J.G. SAGE analysis demonstrates increased expression of TOSO contributing to Fas-mediated resistance in CLL. Blood. 2008 Jul 15;112(2):394-7. 8. Panepucci RA, Calado R.T., Rocha V., Proto-Siqueira R., Silva W.A., Jr., Zago M.A. Higher expression of transcription targets and components of the nuclear factor-kappaB pathway is a distinctive feature of umbilical cord blood CD34+ precursors. Stem Cells. 2007 Jan;25(1):189-96. 9. Proto-Siqueira R., Figueiredo-Pontes L.L., Panepucci RA, Garcia A.B., Rizzatti E.G., Nascimento F.M., Ishikawa H.C., Larson R.E., Falcao R.P., Simpson A.J., Gout I., Filonenko V., Rego E.M., Zago M.A. PRAME is a membrane and cytoplasmic protein aberrantly expressed in chronic lymphocytic leukemia and mantle cell lymphoma. Leukemia research. 2006 Nov;30(11):1333-9. 10. Rizzatti E.G., Falcao R.P., Panepucci RA, Proto-Siqueira R., Anselmo-Lima W.T., Okamoto O.K., Zago M.A. Gene expression profiling of mantle cell lymphoma cells reveals aberrant expression of genes from the PI3K-AKT, WNT and TGFbeta signalling pathways. British journal of haematology. 2005 Aug;130(4):516-26. 11. Panepucci RA, Siufi J.L., Silva W.A., Jr., Proto-Siquiera R., Neder L., Orellana M., Rocha V., Covas D.T., Zago M.A. Comparison of gene expression of umbilical cord vein and bone marrow-derived mesenchymal stem cells. Stem Cells. 2004;22(7):1263-78. 12. Silva W.A., Jr., Covas D.T., Panepucci RA, Proto-Siqueira R., Siufi J.L., Zanette D.L., Santos A.R., Zago M.A. The profile of gene expression of human marrow mesenchymal stem cells. Stem Cells. 2003;21(6):661-9.

Lygia da Veiga Pereira

		Lygia V. Pereira Full Professor of Genetics, IB-USP Head of the National Laboratory of Embryonic Stem Cells (LaNCE) lpereira@usp.br PubMed
Education		1984-1989 Bachelor in Physiscs, Pontifícia Universidade Católica, Rio de Janeiro, RJ. 1989-1990 M.S. in Molecular Biology, Instituto de Biofísica Carlos Chagas Filho, UFRJ, Rio de Janeiro, RJ. 1989-1994 PhD in Human Molecular Genetics, City University of New York, Mount Sinai Graduate School, New York, NY, USA. 2004 Full Professor, University of São Paulo, Brazil.
Research Interests		Human Molecular Genetics Marfan Syndrome Pluripotent Stem Cells Epigenetics – X chromosome inactivation
Research Overview		Major Research Lines: (1) Marfan Syndrome (MFS) – lessons from animal models: MFS is an autosomal dominant disease of the connective tissue that affects the ocular, skeletal and cardiovascular systems. The disease is caused by mutations in the FBN1 gene, that code the fibrillin-1 protein, a major structural component of the extracellular microfibers. By homologous recombination in ES cells, we have established two mouse models for MFS: one containing a FBN1 null allele (mgNull), and another with a deletion of exons 18-25 (mgΔloxPneo). In these models we study the mechanisms of pathogenesis of the disease; genetic and epigenetic factors modifying the phenotype, and we try new therapies for MFS. (2) Epigenetic inheritance – X chromosome inactivation (XCI): we are particularly interested in the process of maintenance of the inactive state of the X chromosome throughout mitosis in human cells. For that, we have developed functional tests that allow the screening of new genes involved in this process. We also work in the characterization of human embryonic stem cells as an in vitro model for the study of XCI. (3) Establishment of new lines of human embryonic stem cells (hESC): Our group is involved in the derivation of new lines of hESC from the Brazilian population, for use in research and therapy. Our interest is to study the epigenetic state of these cells cultured/derived in different conditions. (4) Analysis of the role of GBA mutations in the development of Parkinsonism using induced pluripotent stem cells (iPSC): Recent works have shown a higher frequency of Gaucher disease causing GBA mutations in patients with Parkinsonism (PD). This projects aims the establishment of iPSC lines from patients with PD and mutations in the GBA gene, where we will be able to study the molecular mechanisms behind this association. (5) National Laboratory of Emrbyonic Stem Cells (LaNCE): this is one of eight Centers for Cell Technology sponsored by the Minsitries of Health, and Science and Technology for the production of stem cells under GMP conditions. LaNCE’s specific mission is to promote research and therapy with pluripotetn stem cells in Brazil. This is a project in collaboration with Prof. Stevens Rehen’s group, at UFRJ.
Lab Staff		Molecular Genetics Group Erica Sara M.S. , Student/FAPESP Fernando Sábio M.S., Student/FAPESP Gustavo Ribeiro Fernandes, PhD Student/FAPESP Fernanda Silvestre, PhD Student/CAPES Ana Maria Fraga, PhD Student/FAPESP Cynthia Quinderé Cardoso, PhD Student/CNPq Joana Carvalho Moreira de Mello, PhD Student/FAPESP Naja Vergani, PhD Student/CAPES Giovana Pirolla Cardozo, PhD Student/FAPESP Simone Fonseca, Post-Doc/CAPES Bruno Lazzari Lima, Post-Doc/FAPESP Mariana Morato Marques, Technical support Ana Patrícia Kamisaki Santana, Administrative support
Collaborators		Egberto Reis Barbosa, PhD Universidade de São Paulo, Faculdade de Medicina da Universidade de São Paulo, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo. Av. Dr. Enéas de Carvalho Aguiar, 255 Cerqueira César 05403-000 – Sao Paulo, SP – Brasil Telefone: (11) 30696401 Fax: (11) 30827548 http://www.fm.usp.br/ Silvia Maria Gomes Massironi, PhD Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Imunologia. AV. LINEU PRESTES 1730 CIDADE UNIVERSITARIA 05508-900 – Sao Paulo, SP – Brasil Telefone: (11) 30917398 Fax: (11) 30917224
International Collaborators		Jeanne Loring, PhD Professor of Developmental Neurobiology Director of the Center for Regenerative Medicine Department of Chemical Physiology California Campus Laboratory Website jloring@scripps.edu (858) 784-7767
Facilities Coordination		GMP facility for cell culture
Selected Publications		FRAGA AM, ARAÚJO ESS, STABELLINI R, VERGANI N, PEREIRA LV. A Survey of Parameters Involved in the Establishment of New Lines of Human Embryonic Stem Cells. Stem Cell Reviews, v.1, p.1 – , 2011.AMPS K, ANDREWS PW, ANYFANTIS G, ARMSTRONG L, AVERY S, BAHARVAND H, BAKER J, BAKER D, MUNOZ MB, BEIL S, BENVENISTY N, BEN-YOSEF D, BIANCOTTI JC, BOSMAN A, BRENA RM, BRISON D, CAISANDER G, CAMARASA MV, CHEN J, CHIAO E, CHOI YM, CHOO ABH, COLLINS D, COLMAN A, CROOK J M, DALEY GQ, DALTON A, DE SOUSA PA, DENNING C, DOWNIE J, DVORAK, MONTGOMERY KD, FEKI A, FORD A, FOX V, FRAGA AM, FRUMKIN T, GE L, GOKHALE PJ, GOLAN-LEV T, PEREIRA, LV. et al. Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. Nature Biotechnology, 29:1132–1144, 2011.Fraga, A.M. ; Soukoyan, M. ; Rajan, P ; de Almeida Ferreira Braga, Daniela ; IACONELLI JR., A. ; José Gonçalves Franco Jr ; BORGES JR., E. ; Pereira, L.V. . Establishment Of A Brazilian Line Of Human Embryonic Stem Cells In Defined Medium – Implications For Cell Therapy In An Ethnically Diverse Population. Cell Transplantation, v. 1, p. 1, 2010.Moreira de Mello, Joana Carvalho ; Araújo, Érica Sara Souza de ; Stabellini, Raquel ; Fraga, Ana Maria ; Souza, Jorge Estefano Santana de ; Sumita, Denilce R. ; Camargo, Anamaria A. ; Pereira, Lygia V. . Random X Inactivation and Extensive Mosaicism in Human Placenta Revealed by Analysis of Allele-Specific Gene Expression along the X Chromosome. Plos One, v. 5, p. e10947, 2010.E. Sidransky, et al. Multicenter Analysis of Glucocerebrosidase Mutations in Parkinson’s Disease. New England Journal of Medicine, 361:1651-61, 2009.Raquel Stabellini, Joana Carvalho Moreira de Mello, Lys Molina Hernandes, Lygia V. Pereira. MAOA and GYG2 are submitted to X chromosome inactivation in human fibroblasts. Epigenetics, 4:1-8, 2009.VASQUES, L.R., STABELINI, R., SUKOYAN, M., E PEREIRA, L.V. XIST repression in the absence of DNMT1 and DNMT3B. DNA Research, 12:373-378, 2005.CARTA C, PEREIRA L, ARTEAGA-SOLIS E, LEE-ARTEAGA SY, STARCHER B, MERKEL CA, SUKOYAN M, KERKIS A, HAZEKI N, KEENE DR, SAKAI LY E RAMIREZ F. Fibrillins 1 and 2 perform partially overlapping roles during aortic development. Journal of Biological Chemistry 281(12):8016-8023, 2005.XUE, F., TIAN, C., DU, F., KUBOTA, C., TANEJA, M., DINNYES, A., DAI, Y., LEVINE, H., PEREIRA, L.V., E YANG, X. Aberrant patterns of X chromosome inactivation in bovine clones. Nature Genetics, vol. 31(6):216-220, 2002.Sukoyan, M.A., Kerkis, A.Y., Mello, M.R.B., Kerkis, I.E., Visintin, J.A., Pereira, L.V. Establishment of new murine embryonic stem (ES) cell lines for the generation of mouse models for human genetic diseases. Braz. J. Med. Biol. Res., vol. 35(5):535-542, 2002.Vasques, L.R., and Pereira, L.V. Allele-specific X-linked gene activity in normal human cells assayed by expressed single nucleotide polymorphisms (cSNPs). DNA Research, vol. 8(4):173-177, 2001.

Lewis Joel Greene

		Lewis Joel Greene Full Professor of Cell and Molecular Biology (retired) FMRP/USP Supervisor of the Center for Protein Chemistry, at Regional Blood Center of Ribeirão Preto Volunteer head teacher (senior associate) of FMRP/USP ljgreene@fmrp.usp.br PubMed Scopus ResearchID Scielo Scholar
Education		1951-1955 B.A (magna cum laude), Amherst College, Amherst Massachusetts 1955-1962 Ph D, Rockefeller University, C.H.W. HIRS and G.E. Palade (Research advisors) 1990 Full Professor, University of São Paulo, Brazil
Research Interests		Structure and function of proteins Protein- protein interactions Proteomics as an entry to cell biology Molecular basis of cancer Molecular basis of sepsis
Research Overview		The recent focus of our group has been to understand the molecular mechanisms involved in the development of diseases and cancer . We are collaborating with biologists, clinicians and chemists using the methodologies of protein chemistry and molecular biology to identify biomarkers for diagnosis and prognosis and to understand the processes involved.
Lab Staff		José César Rosa, PhD – Laboratory Collaborator
Collaborators		Carolina Hassibe Thomé, Pos Doc Germano Aguiar Ferreira, Doctoral Student José César Rosa Faculdade de Medicina de Ribeirão Preto – Universidade de São Paulo Centro de Química de Proteínas, Hemocentro de Ribeirão Preto, SP jcrosa@fmrp.usp.br Andréia Machado Leopoldino Departamento de Análises Clínicas, Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo andreiaml@usp.br Dimas Tadeu Covas Faculdade de Medicina de Ribeirão Preto – Universidade de São Paulo Centro Regional de Hemoterapia de Ribeirão Preto dimas@fmrp.usp.br Vitor Marcel Faça Prof. Dr. da Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo Department of Biochemistry and Immunology vitor.faca@fmrp.usp.br Eduardo Magalhães Rego Departamento de Clínica Médica da Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo edumrego@hotmail.com Wilson Araújo da Silva Júnior Departamento de Genética da Faculdade de Medicina de Ribeirão Preto – Universidade de São Paulo wilsonjr@usp.br Roger Chammas Professor Titular, Faculdade de Medicina, Universidade de São Paulo – USP Departamento de Radiologia rchammas@lim24.fm.usp.br
International Collaborators
Facilities Coordination		Mass Spectrometry and Protein Chemistry Lab
Selected Publications		1. SOBRAL, L. M. ; SOUSA, L.O. ; COLETTA, R. D. ; CABRAL, H. ; GREENE, L. J. ; TAJARA, E.H. ; GUTKIND, J. S. ; CURTI, C. ; Leopoldino, A. M. . Stable SET knockdown in head and neck squamous cell carcinoma promotes cell invasion and the mesenchymal-like phenotype in vitro, as well as necrosis, cisplatin sensitivity and lymph node metastasis in xenograft tumor models. Molecular Cancer , v. 13, p. 32, 2014. 2. Thomé, C. H. ; DOS SANTOS, G. A. ; FERREIRA, G. A. ; SCHEUCHER, P. S. ; IZUMI, C. ; Leopoldino, A. M. ; SIMAO, A. M. ; CIANCAGLINI, P. ; DE OLIVEIRA, K. T. ; CHIN, A. ; HANASH, S. M. ; FALCAO, R. P. ; REGO, E. M. ; Greene, L. J. ; Faça, V. M. . Linker for Activation of T-cell Family Member2 (LAT2) a Lipid Raft Adaptor Protein for AKT Signaling, Is an Early Mediator of Alkylphospholipid Anti-leukemic Activity. Molecular & Cellular Proteomics , v. 11, p. 1898-1912, 2012. Citações: 3\| 4 3. Fontes, A. M. ; Melo, F.U.F. ; Greene, L.J. ; Faça, V. M. ; Lin, Y. ; Gerson, S.L. ; Covas, D. T. . Production of human factor VIII-FL in 293T cells using the bicistronic MGMT(P140K)-retroviral vector. Genetics and Molecular Research , v. 11, p. 775-789, 2012. 4. Miranda, H. C. ; HERAI, R. H. ; Thomé, C. H. ; Gomes, G. G. ; PANEPUCCI, R. A. ; ORELLANA, M. D. ; Covas, D. T. ; Muotri, A. R. ; Greene, L.J. ; Faça, V. M. . A quantitative proteomic and transcriptomic comparison of human mesenchymal stem cells from bone marrow and umbilical cord vein. Proteomics (Weinheim. Print) , v. 12, p. 2607-2617, 2012. Citações: 3\| 6 5. Spiller, F. ; FERREIRA, S. H. ; Greene, L. J. ; Rosa, J. C. ; Altruda, F. ; Souto, F. O. ; Hirsch, E. ; Mestriner, F. L. A. C. ; Cunha, F. Q. ; Tolosano, E. ; Alves-Filho, J. C. ; Laure, H. J. ; Freitas, A. ; Costa, C. ; Vinchi, F. . Inhibition of Neutrophil Migration by Hemopexin Leads to Increased Mortality Due to Sepsis in Mice. American Journal of Respiratory and Critical Care Medicine , v. 183, p. 922-931, 2011. Citações: 7\| 7 6. Santos, G. A. S. ; Thomé, C. H. ; FERREIRA, G.A. ; YONEDA, J. S. ; NOBRE, T. M. ; Daghastnali, K.R.P. ; SCHEUCHER, P. S. ; TEIXEIRA, H. L. ; Constantino, M. G. ; DE OLIVEIRA, K. T. ; Faça, V. M. ; FALCAO, R. P. ; GREENE, L. J. ; REGO, E. M. ; CIANCAGLINI, P. . Interaction of 10-(octyloxy) decyl-2-(trimethylammonium) ethyl phosphate with mimetic membranes and cytotoxic effect on leukemic cells. Biochimica et Biophysica Acta. Biomembranes , v. 1798, p. 1714-1723, 2010. Citações: 4\| 5 7. Mestriner, F. L. A. C. ; Spiller, F. ; Laure, H. J. ; Souto, F. O. ; TAVARES-MURTA, B.M. ; Rosa, J. C. ; Basile Filho, A. ; FERREIRA, S. H. ; GREENE, L. J. ; Cunha, F. Q. . Acute-phase protein α -1-acid glycoprotein mediates neutrophil migration failure in sepsis by a nitric oxide-dependent mechanism.. Proceedings of the National Academy of Sciences of the United States of America (Online) , v. 104, p. 19595-19600, 2007. Citações: 20\| 22 8. SOUZA, G. A. ; GODOY, L.M.F. de ; TEIXEIRA, V. R. ; OTAKE, A. H. ; SABINO, A. ; Rosa, J. C. ; Dinarte, A. R. ; Pinheiro, D. G. ; Silva Jr, W. A. ; EBERLIN, M. N. ; Chammas, R. ;GREENE, L. J. . Proteomic and SAGE profiling of murine melanoma progression indicates the reduction of proteins responsible for ROS degradation. Proteomics, Inglaterra, v. 6, n.5, p. 1460-1470, 2006. Citações: 22\| 22

Flávio Vieira Meirelles

		Flávio V. Meirelles Full Professor of Cell Biology & Development, FZEA/USP Member of the Coordinating Committee (Veterinary Medicine) of FAPESP Associate editor of Genetics And Molecular Research meirellf@usp.br PubMed Scopus ORCID ResearcherID Scholar
Education		1989-1993 Graduated in Veterinary Medicine, São Paulo State University, Brazil 1994-1996 Master Thesis in Reproduction, University of Montreal, Canada 1997-1999 PhD in Genetics, Medical Scholl of Ribeirão Preto, University of São Paulo 2004 Full Professor, University of São Paulo, USP, Brazil
Research Interests		Cytoplasmic inheritance Nuclear Reprograming Animal Genetics Mitochondrial genome Embryonic genome activation
Research Overview		Since the last 20 years we have focused on understanding the process of cytoplasmic inheritance. We have successfully created animal models to study mtDNA inheritance, and its related cell death process during early embryo development, which allowed us to develop a patent on SCNT animal production. We also dedicated to understand the process of cytoplasmic inheritance and its effect on nuclear reprograming, especially on the imprinting modifications after in vitro manipulation such as cloning or reprograming somatic cells. Recently we developed an interesting method for depletion of specific organelles or compounds in oocytes, zygotes or cells that allowed us to test for their effect on embryogenesis and to introduce other organelles or compounds (we did it on mitochondria and we are now testing on lipid granules) in order to partially control their inheritance. Within the next 11 years, we will together with the CTC group continue to work on this area willing to evaluate the production of reprogrammed cells to autologous cell therapy and to control the cytoplasmic inheritance in order to apply to cell based therapy.
Lab Staff		Pos Docs Marcos Roberto Chiaratti, PhD Lilian de Jesus Oliveira,PhD José Rodrigo V. Pimentel, PhD PhD Fabiana Fernandes Bressan,MSc Reno Roldi Araujo, MSc Rodrigo Barreto, MSc Rafael Sampaio, MSc Juliano Sangalli, MSc Pedro Ratto Pires, MSc MSc Lais Vicari Pessoa, DVM Gabriella Mamede Andrade, DVM
Collaborators		Claudia Lima Verde Leal, PhD Universidade Federal de São Paulo LMMD Carlos Eduardo Ambrósio, PhD Universidade Federal de São Paulo LMMD Felipe Perecin, PhD Universidade Federal de São Paulo LMMD Heidge Fukumasu Universidade Federal de São Paulo LMMD Maria Angélica Miglino Universidade Federal de São Paulo FMVZ – Programa de Pós Graduação em Anatomia
International Collaborators		Joanna Poulton, PhD Professor of Genetics Univ. of Oxford, Lawrence Charles Smith, PhD Full Professor – Canadian Chair of Animal Cloning Université de Montréal Willian Allan King Full Professor – Canadian Chair of Animal Biotechnology Guelph – ON Canada
Facilities Coordination		Embryo Manipulation Facilities including IVF Laboratory, Cell sorting, Basic Molecular Biology lab. Animal Hospital Facility dedicated for Innovative Therapy.
Selected Publications		1. BRESSAN, Fabiana Fernandes ; Dos Santos Miranda, Moyses ; PERECIN, Felipe ; De Bem, Tiago Henrique ; Pereira, Flavia Thomaz Verechia ; Russo-Carbolante, Elisa Maria ; Alves, Daiani ; Strauss, Bryan ; Bajgelman, Marcio ; Krieger, José Eduardo ; Binelli, Mario ; Meirelles, Flavio Vieira . Improved Production of Genetically Modified Fetuses with Homogeneous Transgene Expression After Transgene Integration Site Analysis and Recloning in Cattle. Cellular Reprogramming (Formerly Cloning and Stem Cells), v.13 (1) p.29-36, 2011. 2. CHIARATTI, Marcos Roberto; FERREIRA, Christina Ramires; PERECIN, Felipe … Meirelles, Flávio Vieira . Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. Reprod. BioMedicine Online, v. 22, p. 172-183, 2011. 3. Suzuki Jr, João ; Therrien, Jacinthe ; Filion, France ; Lefebvre, Rejean …Perecin, Felipe ; Meirelles, Flávio V. ; Smith, Lawrence Charles . Loss of Methylation at H19 DMD Is Associated with Biallelic Expression and Reduced Development in Cattle Derived by Somatic Cell Nuclear Transfer. Biology of Reproduction, v. 84, p. 947-956, 2011. 4. Bressan, Fabiana Fernandes; Perecin, Felipe; Sangalli, Juliano Rodrigues ; Meirelles, F. V. Reprogramming somatic cells: pluripotency through gene induction and nuclear transfer. Acta Scientiae Veterinariae, v. 39, p. s83-s95, 2011. 5. De Bem, T. H. C. ; Chiaratti, M. R. ; Rochetti, R. ; Bressan, F. F. ; Sangalli, J. R. ; Miranda, M. S. ; Pires, P. R. L. ; Schwarz, K.R.L. ; Sampaio, R. V. ; Fantinato Neto, P. ; Pimentel, J. R. V. ; Perecin, F. ; Smith, L. C. ; Meirelles, F. V. ; Leal, C. L. V. . Viable calves produced by somatic nuclear transfer using meiotic-blocked oocytes. Cellular reprogramming, v. 13, p. DOI: 10.1089/c, 2011. 6. Chiaratti, M R ; Meirelles, F. V. ; Wells, D ; Poulton, J . Therapeutic treatments of mtDNA diseases at the earliest stages of human development. Mitochondrion (Amsterdam. Print), v. 11, p. 820-828, 2011. 7. Ferreira, C. R. ; Burgstaller, J. P. ; Perecin, F. ; Garcia, J. M. ; Chiaratti, M. R. ; Meo, S. C. ; Muller, M. ; Smith, L. C. ; Meirelles, F. V. Pronounced Segregation of Donor Mitochondria Introduced by Bovine Ooplasmic Transfer to the Female Germ-Line. Biology of Reproduction, v. 82, p. 563-571, 2010. 8. Meirelles, Flávio V. ; Birgel, Eduardo H. ; PERECIN, Felipe Et Al., Delivery of cloned offspring: experience in Zebu cattle. Reprod., Fertility and Dev. v. 22, p. 88, 2010. 9. Chiaratti, M. R. ; Meirelles, F. V. . Mitochondrial DNA Copy Number, a Marker of Viability for Oocytes. Biology of Reproduction, v. 83, p. 1-2, 2010. 10. 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