Elisa Maria de Sousa Russo Carbolante

		Elisa Maria de Sousa Russo Carbolante Professor at FCFRP/USP elisa@fcfrp.usp.br View a complete PubMed search
Education		1988-1991 Graduated in Pharmacy, University of São Paulo, Brazil 1996-1998 Master Thesis in Biochemistry, Medical School of Ribeirão Preto, University of São Paulo 1998-2002 PhD in Biochemistry, Medical School of Ribeirão Preto, University of São Paulo 2003-2006 Postdoctoral Research Fellow, Blood Center of Ribeirão Preto, University of São Paulo
Research Interests		iPS Recombinant Protein Genetic modification of cells
Research Overview		Our group has two main focuses which are the expression of recombinant proteins and the reprogramming of cells. The proteins are expressed in our laboratory to be used as therapeutic drugs (FVIII and FIX), in diagnostic tests (HIV and HTLV) and also to reprogram cells (oct4, sox2, KLF4). We have reprogrammed fibroblasts to iPS cells using the genetic modification approach employing a combination of transcription factors, delivered by lentivirus, different from the classic one (OSKM). Now we are interested in reprogram the cells without the genetic modification and for this purpose we are going to use the direct modification of cells (from fibroblasts to hematopoietic cells) and also we are expressing the transcription factors involved in the manutence of pluripotency to reprogram the cells using these proteins.
Lab Staff		Recombinant, Protein Group Aline Bomfim, Post graduation Student Ana Paula de Pontes, Post graduation Student Florencia Tellechea, Post graduation Student Cell Modification Group Luiza Cunha Junqueira Reis, Post graduation Student Fillipe Luiza Rosa, Post graduation Student Virgínia Picanço Castro, phD
Collaborators		Angelo A Cardoso, PhD Universidade de Purdue-Indiana, EUA Lygia Veiga Pereira Instituto de Biociências – USP Flávio Meirelles FMVZ – USP, Pirassununga
International Collaborators
Facilities Coordination
Selected Publications		1. BRESSAN, F.F. ; Miranda, M ; Perecin, F ; BEM, T. H. C. ; PEREIRA, F. T. V. ; RUSSO-CARBOLANTE, EMS ; Alves, DCC ; Strauss, B ; Bajgelma, M ; Krieger, JE ; Binelli, M ; Meirelles FV . Improved production of genetically modified fetuses with homogeneous transgene expression after transgene integration site analyses and recloning in catle. Cellular Reprogramming, v. 13, p. 29-36, 2011. 2. SWIECH, K. ; Kamen, A ; Ansorge, S ; Durocher, Y ; Picanço-Castro, Virgínia ; RUSSO-CARBOLANTE, EMS ; COVAS, D. T. . Transient transfection of serum-free suspension HEK 293. BMC Biotechnology (Online), v. 11, p. 114, 2011. 3. Picanço-Castro, Virginia ; RUSSO-CARBOLANTE, EMS ; Reis, Luiza Junqueira; Fraga, Ana Maria ; Orellana, MD ; ORELLANA md ; Pereira, Lygia Veiga ; Covas, Dimas Tadeu . Pluripotent reprogramming of fibroblasts by lentiviral-mediated insertion of SOX2, C-MYC and TCL-1A. Stem Cells and Development, v. 20, p. 169-180, 2011. 4. Russo-Carbolante, Elisa Maria ; Picanço-Castro, Virgínia ; Alves, Daiani Cristina Cilão ; Fernandes, Andrielle Castilho ; Almeida-Porada, Graça ; Tonn, Torsten ; Covas, Dimas Tadeu . Integration pattern of HIV-1 based lentiviral vector carrying recombinant coagulation factor VIII in Sk-Hep and 293T cells. Biotechnology Letters, v.33, p. 23-31, 2010. 5. Picanço-Castro, V ; RUSSO-CARBOLANTE, EMS ; FONTES, A. M. ; Fernandes, AC ; COVAS, D. T. . An enhancer/promoter combination strengthens the expession of blood-coagulation factor VIII in non-viral expression vectors. Genetics and Molecular Research, v. 7, p. 314-325, 2008. 6. Picanço-Castro, V ; FONTES, A. M. ; RUSSO-CARBOLANTE, EMS ; COVAS, D. T. . Lentiviral-mediated gene transfer – a patent review. Expert Opinion on Therapeutic Patents, v. 18, p. 1-15, 2008. 7. RUSSO-CARBOLANTE, EMS ; PENTEADO, F. L. ; MEDEIROS, L. ; KASHIMA, S. ; TAKAYANAGUI, O ; COVAS, D. T. . Cloning and expression of the transmembranic glycoprotein from human T cell lymphotropic virus in a prokaryotic system. Revista da Sociedade Brasileira de Medicina Tropical, v. 40, p. 277-81, 2007. 8. LUCISANO-VALIM, Y. M. ; KABEYA, L. M. ; KANASHIRO, A. ; RUSSO-CARBOLANTE, EMS ; POLIZELLO, A. C. M. ; AZZOLINI, A. E. C. S. ; SILVA, S. C. ; LOPES, J. L. C. ; OLIVEIRA, C. A. ; MANTOVANI, B. . A simple method to study the activity of natural compounds on the chemiluminescence of neutrophils upon stimulation by immune complexes. J Pharmacol and toxicol methods, v. 47, p. 53-58, 2002. 9. Picanço-Castro, Virginia ; RUSSO-CARBOLANTE, EMS . Reprogramming of Human Fibroblasts into Pluripotent cells: Role of lentiviral mediated transcription factors. In: M.A. Hayat. (Org.). Series Stem Cells and Cancer Stem cells. Vol 5 Therapeutic Applications in Disease and Injury. : Springer, 2011, v. 5, p. -.